35份重庆大茶树种质资源遗传多样性的SSR分析

Analysis of Genetic Diversity in 35 Ancient Tea Accessions from Chongqing with SSR Markers

茶树是中国重要的经济植物,遗传多样性分析对于其种质资源的评价与保存十分重要。本研究采用SSR分子标记技术对35份重庆大茶树种质资源进行遗传多样性分析,用梯度PCR仪对22对SSR引物进行扩增筛选,获得21对多态性引物。用21对引物对35份大茶树种质扩增,经8%非变性聚丙烯酰胺电泳检测,共扩增出48个条带,多态性条带38个,多态带百分率为79.17%。35份种质的遗传距离在0.313~0.955之间。UPGMA聚类分析显示,35份重庆大茶树种质在遗传相似系数为0.702时可分为5个聚类,亲缘关系最近的是“南川1号”和“南川2号”。

Tea plant is an economically valuable species in China.The genetic diversity of tea plant is very important for the evaluation and conservation of germplasm resources.Based on SSR molecular markers,the genetic diversity of 35 ancient tea germplasm resources in Chongqing was analyzed in this study,22 pairs of SSR primers were screened by gradient PCR instrument,and the primers with high polymorphism were selected.21 pairs of primers were proved to be polymorphic from 22 pairs of SSR primers,a total of 48 bands were generated,among which 38(79.17%)were polymorphic.The genetic distance values ranged from 0.313 to 0.955.The UPGMA result indicated that 35 ancient tea germplasm resources were clustered into five groups at a threshold of 0.702,and"Nanchuan 1"and"Nanchuan 2"were clustered closely.