实时荧光定量聚合酶链式反应在获得性免疫缺陷综合征合并水痘-带状疱疹病毒感染中的应用

Value of real-time fluorescent quantitative polymerase chain reaction in acquired immunodeficiency syndrome complicated with varicella-zoster virus infection

目的研究水痘-带状疱疹病毒(VZV)核酸检测技术对获得性免疫缺陷综合征(AIDS)合并带状疱疹的诊断价值.方法收集AIDS合并带状疱疹患者的血液和疱疹液,进行病毒DNA检测,同时进行血清抗-VZV IgM及T细胞亚群检测.结果纳入AIDS合并带状疱疹患者共32例,入组患者疱疹液VZV DNA检测均为阳性,其中28例患者血液VZV DNA检测为阳性;仅有7例AIDS患者血清抗-VZV IgM阳性;疱疹液VZV DNA载量显著高于血液VZV DNA水平,差异有统计学意义(t=3.173、P=0.0032),血液及疱疹液VZV DNA检测阳性率显著高于血清抗-VZV IgM检测阳性率(87.5%、100%vs.21.8%,P<0.001).结论 VZV核酸检测技术对于AIDS合并水痘-带状疱疹病毒感染的病原学诊断具有重要价值.

Objective To investigate the diagnostic value of varicella zoster virus (VZV) nucleic acid detection in acquired immunodeficiency syndrome (AIDS) complicated with herpes zoster. Methods The blood and herpes fluid of AIDS patients with herpes zoster were collected, the virus DNA was detected, and the serum anti-VZV IgM and T cell subsets were detected, respectively. Results A total of 32 patients with AIDS complicated with herpes zoster were included, all patients with VZV DNA positive in herpetic fluid, 28 patients with blood VZV DNA positive;only 7 AIDS patients with serum anti-VZV IgM positive, and the load of VZV DNA in herpetic fluid was significantly higher than that in blood, with significant difference (t = 3.173, P = 0.0032). The positive rate of VZV DNA in blood and herpetic fluid were significantly higher than that in serum anti-VZV IgM, with significant difference (87.5%, 100% vs. 21.8%;P < 0.001). Conclusion VZV nucleic acid detection was of great value in the etiological diagnosis of AIDS complicated with varicella zoster virus infection.