基于iPBS标记的玫瑰香系葡萄遗传多样性分析与指纹图谱构建

Genetic diversity analysis and fingerprinting construction of Muscat Hamburg grapevine series by iPBS marker

为探讨玫瑰香系葡萄种质资源间的遗传关系,利用iPBS标记对39个玫瑰香系葡萄品种进行遗传多样性分析,并构建其DNA指纹图谱。筛选出14个引物,对39个玫瑰香系葡萄品种进行PCR扩增,共获得152条带,其中,多态性条带132条,多态性比率为86.86%。各引物多态性信息含量(PIC)、观测等位基因数(Na)、有效等位基因数(Ne)、Neis基因多样性指数(H)和Shannons信息指数(I)的平均值分别为0.8638、1.8684、1.4090、0.2518和0.3907。39个玫瑰香系葡萄品种间的遗传相似系数为0.5263~0.9408,变幅为0.4135。上述结果表明:39个玫瑰香系葡萄品种间具有较丰富的遗传多样性。利用UPGMA构建39个玫瑰香系葡萄种质资源的聚类树状图,在遗传相似系数(GS)为0.72处可将39个玫瑰香系葡萄品种分为4组,其中欧亚种主要分布在第Ⅰ组,而欧美杂交种主要分布在第Ⅱ、Ⅲ和Ⅳ组。利用8个引物的16个多态性位点构建了39个玫瑰香系葡萄品种的DNA指纹图谱,该图谱可为玫瑰香系葡萄品种的鉴定提供科学依据。

In order to explore the genetic relationship among the germplasm resources of Muscat Hamburg grapevine series, the genetic diversity of 39 cultivars was analyzed by iPBS marker, and their DNA fingerprints were constructed. 14 primers were screened for PCR amplification of 39 cultivars. A total of 152 bands were generated, of which 132 bands were polymorphic bands(86.86%).The average value of polymorphism information content, number of observed alleles, number of effective alleles, Nei’s gene diversity index and Shannon’s information index were 0.863 8, 1.868 4, 1.409 0, 0.251 8 and 0.390 7, respectively. The genetic similarity coefficient among 39 cultivars ranged from 0.526 3 to 0.940 8 with a range of 0.413 5. The above results indicated that there were rich genetic diversity among 39 cultivars. The dendrogram of these 39 cultivars was constructed using UPGMA. They could be divided into 4 groups at genetic similarity coefficient of 0.72, of which V. vinifera were mainly distributed in Group Ⅰ, while the Euro-American hybrids species were mainly distributed in Groups Ⅱ, Ⅲ and Ⅳ. The DNA fingerprints of 39 cultivars were constructed using 16 polymorphic loci amplified of 8 primers, providing scientific basis for the identification of Muscat Hamburg grapevine series.