一株临沧野生黄鸡■的鉴定与生长条件研究

Identification and culture conditions of a wild-type Termitomyces aurantiacus strain from Lincang

为筛选黄鸡[土從]菌丝生长的最优培养条件,为其仿生栽培奠定基础,从采集于云南临沧的野生鸡子实体中分离纯化获得纯培养物LCT10,结合形态学与ITS序列分析鉴定其分类学地位,并设计了不同培养条件,研究不同温度、pH、光照强度、通气性、碳氮源与碳氮比对野生黄鸡菌丝生长的影响。试验表明,采集到的菌株LCT10为黄鸡[土從](Termitomyces aurantiacus),其形态特征与分子鉴定结果一致。该菌菌丝体在5~30℃均能生长,最适生长温度为25℃;菌丝在pH4.0~12.0均能生长,当pH为8.0,培养皿在黑纸包裹下培养菌丝生长速度最快;在5种通气处理下均能生长,不做任何通气处理的菌丝生长最快。能利用可溶性淀粉、玉米粉、蔗糖、蜂蜜、葡萄糖作为碳源,蛋白胨、甘氨酸、硝酸铵、尿素作为氮源,其中,在蜂蜜上生长浓密且生长快,最适氮源为蛋白胨,最适C/N为(20~30)∶1。LCT10在最适培养条件下的生长情况与其他处理相比有显著差异(P<0.05)。

To screen the optimal culture condition for mycelial growth of Termitomyces aurantiacus and lay the foundation for its bionic cultivation,strain LCT10 was isolated from a wild mushroom fruiting body of Termitomyces from Lincang,Yunnan. Taxonomic status was identified based on its morphology and ITS sequence analysis,and the different culture conditions were designed to determine the effect of physical factors (temperature,light intensity and aeration) and chemical factors (pH,carbon resources,nitrogen sources and C/N) on mycelial growth of T.aurantiacus.The result showed that the strain LCT10 was T.aurantiacus,its morphological characteristics were consistent with its molecular identification.Its optimal temperature and pH were25 ℃ and 8.0.It was able to grow in 5 kinds of ventilation treatments, and the growth of mycelia was the fastest in the treatment of no ventilation.Soluble starch,corn flour,sucrose,honey and glucose were suitable for its growth,honey was the best carbon soure for mycelial growth.Peptone,glycine,ammonium nitrate and urea could be used as nitrogen sources and peptone was the best.The optimum C/N for mycelial growth was (20-30)∶1.The growth of strain LCT10 under the best culture conditions had significant difference at level of 0.05 comparing with other treatments.