L-精氨酸在实验室果蝇扩繁中的应用

Application of L-arginine in laboratory Drosophila melanogaster propagation

目的实验旨在研究在玉米琼脂培养基中添加L-精氨酸,探究L-精氨酸对果蝇化蛹时间、羽化时间、羽化率及雌雄比例的影响。方法实验分为2组,分别为对照组和实验组,对照组为基础培养基,实验组在基础培养基加1%的L-精氨酸,每管中雌、雄1∶1放入7对果蝇,每组12个重复,接种72h后移除亲本,统计各组果蝇化蛹时间、羽化时间、羽化数及各组雌雄个数。结果培养基中添加1%的L-精氨酸,白眼(w1118)、残翅(CyO)和野生型黑腹果蝇的化蛹数、羽化数分别比对照组提高313.64%、303.53%(P<0.01),157.09%、182.76%(P<0.01)和233.33%、239.03%(P<0.01),添加1%的L-精氨酸后白眼和野生型果蝇的化蛹时间比对照组提前2d。结论在本实验条件下,1%的L-精氨酸可提高实验室果蝇繁殖力,可应用于实验室果蝇的扩繁。

Objective To explore the effect of L-arginine on pupation time, eclosion time, eclosion rate and male/female ratio in Drosophila melanogaster. Methods Two groups of fruit flies were included in this study, the control group received corn meal agar medium(basic medium) and the experimental group received 1% L-arginine added in the basal medium. Seven pairs of fruit flies were placed in each tube, female: male=1∶1, 12 replicates in each group, and the parent flies were removed after 72 h. The time of pupation, the time of feathering, the number of feathering, and the number of males and females in each group were counted. Results After the addition of 1% L-arginine in the basic medium, the numbers of pupation and feathering, and the feathering rate of the white eye(w1118), residual wing(CyO) and wild type Drosophila melanogaster were increased by 313.64% and 303.53%(P< 0.01), 157.09% and 182.76%(P< 0.01), and 233.33% and 239.03%(P< 0.01), respectively. In the group with addition of 1% L-arginine, the pupation time of the both white eye and wild type flies was 2 days earlier than the control group. Conclusions Addition of 1% L-arginine in the basic medium, used under the conditions of this experiment, can increase the fertility of laboratory fruit flies and can be applied to the expansion of laboratory fruit flies.