基于网络药理学的秦艽抗炎活性作用分析

Anti-inflammatory Activity Analysis of Gentiana macrophylla Based on Network Pharmacology

目的:通过网络药理学方法预测秦艽抗炎活性主要成分的作用靶点,构建干预炎症的“活性成分-炎症靶点-抗炎通路”网络关系,预测其干预炎症的靶点及机制。方法:通过中药系统药理学分析平台建立秦艽的化学成分和靶点数据集;OMIM数据库筛选炎症相关的靶点;PPI数据库构建蛋白交互靶点;用Cytoscape软件构建“化学成分-作用蛋白-炎症靶点”交互网络图。筛选出秦艽调节炎症的关键作用靶点,利用DAVID数据库和Cytoscape的插件ClueGO对入选靶点进行KEGG通路富集和GO分析。结果:经筛选和分析获得11个秦艽活性成分,主要为秦艽乙素、秦艽甲素、西藏龙胆碱、α-香树脂醇、秦艽丙素、马钱苷酸等;筛选和分析获得25个抗炎靶点,包括细胞肿瘤抗原p53、NF-kappa-B必需调节剂、肿瘤坏死因子受体相关因子6等,秦艽发挥抗炎作用的可能通路包括细胞凋亡、TNF信号通路、MAPK信号通路等。结论:预测秦艽调节炎症的潜在靶点,揭示其作用机制,为进一步研究秦艽的抗炎机制和临床应用提供参考。

Objective: The network pharmacology method was used to predict the targets of the main anti-inflammatory components of Gentiana macrophylla. The network relationship of "active components- inflammatory targets- anti- inflammatory pathway" was constructed to predict the targets and mechanisms of its intervention in inflammation. Methods: The chemical composition and target datasets of Gentiana macrophylla were established by the pharmacological analysis platform of Chinese medicine system;the OMIM database was used to screen inflammation-related targets;the PPI database was used to construct protein interaction targets;and the "chemical component-functional protein-targets of inflammation" interaction network was constructed using Cytoscape software. The key targets for the regulation of inflammation in Gentiana macrophylla were screened out. The KEGG pathway enrichment and GO analysis were performed on the selected targets using the DAVID database and Cytoscape plug-in Clue GO. Results: 11 active constituents of Gentiana macrophylla were screened and analyzed, including gentianidine, gentianine, gentiatibetine, alpha-amyrin, gentianal and loganic acid. 25 anti-inflammatory targets were screened and analyzed, including cell tumor antigen p53, essential regulator of NF-kappa-B and tumor necrosis factor receptor-related factors 6 and so on. The possible anti-inflammatory pathways of Gentiana macrophylla include apoptosis, TNF signaling pathway, and MAPK signaling pathway. Conclusion: The potential targets of Gentiana macrophylla for regulating inflammation were predicted and the mechanism of action was revealed, which provideed reference for further study on the anti-inflammatory mechanism and clinical application of Gentiana macrophylla.