摘要
目的建立参七脉心通胶囊的质量控制方法。方法采用高效液相色谱法(HPLC),以人参皂苷Rg1作为指标成分进行定量测定。结果色谱条件为:色谱柱:Agilent ZORBAX SB-C18(5μm,4. 6 mm×250 mm);流动相∶乙腈-水(25∶75);检测波长:203 nm;流速:0. 8 m L/min;柱温:25℃;进样量10μL。人参皂苷Rg1进样量在5. 39~50. 30μg/m L范围内与峰面积呈良好线性,相关系数r=0. 999 7。平均加样回收率为99. 70%,RSD=1. 08%。结论所建立的检测方法准确、可靠、专属性强,可作为参七脉心通胶囊的质量控制方法。
Objective This study was established the quality control method of shenqimaixintong capsule. Methods High performance liquid chromatography( HPLC) was used to determine ginsenoside Rg1 quantitatively. Results Chromatographic conditions were as follows: Chromatographic column: Agilent ZORBAX SB-C18( 5 μm,4. 6 mm × 250 mm);Mobile phase: acetonitrile-water( 25 ∶ 75);Detection wavelength: 203 nm;Flow rate: 0. 8 m L/min;Column temperature25 ℃;Injection volume 10 μL. The volume of ginsenoside Rg1 was well linear with peak area in the range of 5. 39 ~ 50. 30μg/m L,and the correlation coefficient r = 0. 9997. The average recovery was 99. 70 %,RSD = 1. 08 %. Conclusion The method is accurate,reliable and specific,which can be used for the quality control of shenqimaixintong capsule.
作者
欧阳勇
余应嘉
梁敏
陈朝俊
OUYANG Yong;YU Yingjia;LIANG Min;CHEN Chaojun(Guangzhou Hospital of Integrated Traditional and West Medicine, Guangzhou 510800, China)
出处
《现代医院》
2019年第9期1377-1379,1382,共4页
Modern Hospitals
基金
广东省中医药局科研项目立项资助(20181215)
