EMI1高表达与结直肠癌临床病理特征及不良预后的相关性

The Correlation between High Expression of EMI1 and Clinicopathological Features and Poor Prognosis of Colorectal Cancer

目的探讨EMI1在结直肠癌中的表达及临床意义。方法 RT-PCR和Western blotting方法检测癌和癌旁组织配对的结直肠癌临床标本中的EMI1 mRNA和蛋白的表达水平,同时,采用免疫组织化学技术检测240例石蜡包埋的结直肠癌标本,统计学分析EMI1表达与临床病理学指标、预后的关系。结果与相应配对的癌旁组织相比,癌组织标本中EMI1 mRNA和蛋白明显上调。统计学分析结果显示,EMI1表达与结直肠癌的临床病理特征密切相关:临床分期(P=0. 004),T分期(P=0. 011),M分期(P=0. 003)。进一步的多因素分析显示,EMI1表达可作为结直肠癌独立的预后因素(P=0. 041,HR:1. 558,95%CI:1. 1019~2. 384)。结论 EMI1在结直肠癌中表达失调升高,EMI1高表达预示结直肠癌患者的不良预后。EMI1可能可以作为结直肠癌的一个新的预后指标。

Objective To investigate the expression and prognostic significance of EMI1 in colorectal cancer. Methods RT-PCR and Western blotting assays were performed to examine EMI1 expression in CRC tissues and matched adjacent non-cancerous tissues. And immunohistochemistry was used to evaluate EMI1 expression in archived 240 paraffin-embedded CRC specimens. Statistical analysis was conducted the relationships between EMI1 expression and clinicopathologic parameters and prognosis of CRC. Results The mRNA and protein levels of EMI1 were up-regulated in CRC tissues compared with their adjacent non-cancerous tissues. The EMI1 expression was closely correlated with clinicopathologic parameters of CRC,clinical stage( P = 0. 004),T classification( P = 0. 011),M classification( P = 0. 003). Furthermore,multivariate analysis demonstrated that EMI1 expression was an independent prognostic factor for CRC( P = 0. 041,HR: 1. 558,95% CI: 1. 1019 ~ 2. 384). Conclusion EMI1 level dyregulated and increased in colorectal cancer. High expression of EMI1 indicated poor prognosis of CRC. EMI1 may serve as a novel prognostic biomarker for CRC.