急性T淋巴细胞白血病患者外周血HES1、C-MYC和NF-kB表达水平及其意义

Expression Levels of HES1, C-MYC and NF-kB in Peripheral Blood of Patients with T Cell Acute Lymphoblastic Leukemia and Their Significance

目的:分析并探讨急性T淋巴细胞白血病(T-ALL)患者外周血HES1,C-MYC和NF-k B表达水平及其意义。方法:2012年6月-2015年3月在本院确诊为T-ALL和急性髓系白血病(AML)患者各60例,分别设为T-ALL组和AML组,另30例健康体检人群作为对照组。采集3组外周血,应用RT-PCR法检测HES1、C-MYC、NF-kB表达水平,对比3组一般资料及不同白血病型、细胞遗传学分型、预后患者外周血HES1、C-MYC、NF-kB的表达差异。结果:3组年龄、性别等基线资料比较无差异(P>0.05)。T-ALL组、AML组血红蛋白(Hb)、白细胞(WBC)、血小板(Plt)、BM细胞(BM blast cell)均显著高于正常对照组(P<0.01),但T-ALL组与AML组之间差异无统计学意义(P>0.05)。3组外周血HES1、C-MYC、NF-kB表达水平差异有显著统计学意义(P<0.01),且在T-ALL组和AML组显著高于对照组(P<0.01),在T-ALL组中HES1、C-MYC、NF-kB表达水平显著高于AML组(P<0.01)。T-ALL组中预后不良患者HES1、C-MYC、NF-kB表达水平均显著高于预后良好的T-ALL患者(P<0.01),完全缓解组HES1、C-MYC、NF-k B表达水平显著高于部分缓解组及未缓解组(P<0.01)。结论:HES1、C-MYC、NF-kB在T-ALL患者外周血中呈高表达状态,其表达水平可因细胞遗传学和疗效而有所差异。

Objective:To analyze and investigate the expression levels of HES1,C-MYC and NF-kB in peripheral blood of patients with T cell acute lymphoblastic leukemia(T-ALL)and their significance.Methods:Sixty patients with T-ALL and 60 patients with acute myelogenous leukemia(AML)diagnosed in our hospital from June 2012 to March 2015 were enrolled in T-ALL group and AML group,respectively.Another 30 healthy people were enrolled in the control group.Peripheral blood was collected to detect the expression levels of HES1,C-MYC and NF-kB by RT-PCR.The general data and the expression of HES1,C-MYC and NF-kB in peripheral blood were compared among the patients with different type of leukemia,cytogenetical types and different prognosis.Results:There was no significant difference in baseline data,such as age and sex among the 3 groups(P>0.05).The Hb level,WBC and Plt count,BM blast cell ratio in T-ALL and AML groups all were significantly higher than those in control group(P<0.01),but there were no statistical difference in above-mentioned indicators between T-ALL and AML groups(P>0.05).The expression levels of HES1,C-MYC and NF-kB in peripheral blood among 3 groups were significantly differenct(P<0.01),the expressions levels of HES1,C-MYC and NF-kB in T-ALL and AML groups were significantly higher than those in control were significantly group(P<0.01),moreover,the expression levels of above-mentional indicators in T-ALL groups were significantly higher than than those in AML group(P<0.01).The expression levels of HES1,C-MYC and NF-kB iin T-ALL patients with poor prognosis were significantly higher than those in T-ALL patients with favorable prognosis(P<0.01);the expression levels of HES1,C-MYC and NF-kB in peripheral blood of patients with different theraptic efficacy were follow:complete remission group<partial remission group<no remission group(P<0.01).Conclusion:The HES1,C-MYC and NF-kB are highly expressed in peripheral blood of the patients with T-ALL,moreover,the expression levels maybe different,because of the cytogenetic,and theraptic efficacy.