槲皮素对血小板凋亡的影响及其机制

Effect of Quercetin on Apoptosis of Platelets and Its Mechanism

目的:探讨槲皮素对储存损伤期间血小板凋亡的影响及其可能的内在机制。方法:通过流式细胞术检测并分析经不同浓度的槲皮素处理2和4 d的血小板的凋亡情况。通过Western blot方法检测槲皮素处理2和4 d的血小板相关凋亡蛋白表达的变化。结果:2、4和8μmol/L槲皮素处理血小板d 2时的凋亡率分别为3.12%±0.32%、2.89%±0.15%、2.31%±0.28%,与对照组相比有显著性差异(P<0.01),且随着槲皮素浓度的增高,血小板的凋亡比率逐渐降低,即槲皮素对其凋亡的抑制有一定的浓度依赖性(r=-0.9985);d 4时亦观察到了类似的结果。2、4和8μmol/L槲皮素处理2 d可促进抗凋亡蛋白BCL-2表达,抑制促凋亡蛋白BAX表达,导致BCL-2/BAX蛋白的比例显著升高(P<0.01),进而抑制血小板的凋亡;d 4时亦观察到了类似的结果。结论:槲皮素可通过上调凋亡相关蛋白BCL-2/BAX比例来抑制血小板凋亡,且对血小板凋亡的抑制有一定的浓度依赖性。

Objective:To investigate the effects of quercetin on the apoptosis of platelets and to analyze the intrinsic mechanism.Methods:Firstly,the effects of quecetin on the apoptosis of platelets was detected by flow cytometry.Secondly,Western blot was used to detect the expression of apoptosis-related proteins in the platelets treated with quercetin for 2 and 4 day.Results:By flow cytometry,it was found that the apoptosis of platelets in the quercetin-treated group(2,4 and 8μmol/L)was inhibited,the apoptosis rate of platelets in 2,4 and 8μmol/L quercetin group was 3.12%±0.32%,2.89%±0.15%and 2.31%±0.28%,respectively,which were signigicantly lover than that in control group(P<0.01).With the increase of quecetin concentration,the proportion ratio of platelets significantly decreased in a concentration-dependent manner(r=-0.9985).Similar results were observed on the 4 th day.Western blot showed that the treatment with quercetin(2,4 and 8μmol/L)promoted the expression of anti-apoptotic protein BCL-2,inhibited the expression of pro-apoptotic protein BAX,resulting in a significant increase in the ratio of BCL-2/BAX(P<0.01),thereby inhibiting the apoptosis of platelets.Similar results were observed on the 4 th day.Conclusion:Quercetin can inhibit platelet apoptosis by increasing the ratio of apoptosis-related protein BCL-2/BAX in a concentration-dependent manner.