摘要
目的 评价H7N9禽流感病毒裂解佐剂(MF59)疫苗的长期免疫原性。方法 制备含MF59佐剂的H7N9禽流感病毒裂解疫苗成品,放置于(6±2)℃环境下,取保存6、24和30个月后的疫苗,对小鼠进行免疫,以血凝抑制效价和微量中和抗体滴度来评估该疫苗的免疫原性。同时,用存放30个月的疫苗进行2次免疫后,对小鼠进行攻毒,观察MF59佐剂疫苗的免疫保护效应。结果 H7N9禽流感病毒裂解佐剂(MF59)疫苗在(6±2)℃保存30个月后免疫小鼠,检测其血凝抑制抗体效价和微量中和抗体滴度均没有明显的变化,且免疫小鼠能够有效抵御H7N9病毒的感染及其致病效应。结论 H7N9禽流感病毒裂解佐剂(MF59)疫苗具有良好的免疫原性,在(6±2)℃至少可保存30个月。
Objective To evaluate the long-term immunogenicity for the avian influenza A H7N9 split vaccine with the MF59 adjuvant.Methods Preparation the vaccine products was combined with MF59 adjuvant, then stored at(6±2)℃ environment.Mice were immunized with the vaccines stored for 6, 24,30 months.The stability for immunogenicity of the vaccine was invastigated, and the antibody response index was evaluated in terms of the hemagglutination inhibition and micro-neutralization titers.At the same time, after priming-boosting immunization by the vaccine with MF59 adjuvant stored for30 months, the mice were challenged with ZJU01strain to observe the efficacy from the vaccine.Result There were no a significant change occurred in the hemagglutination inhibition and micro-neutralization titers from the vaccine stored for30 months.The immunized mice could effectively resist the infection of H7N9 virus and related pathogenic effect.Conclusion The test showed that the immunogenicity for H7N9 split vaccine with MF59 maintained in a consistant state, and the vaccine could be stored at (6±2)℃ for more than30 months.
作者
吴晓鑫
沈吉友
李方仲
姚航平
姚伟
李兰娟
WU Xiao-xin;SHEN Ji-you;LI Fang-zhong;YAO Hang-ping;YAO Wei;LI Lan-juan(Department of Infection, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou310003, Zhejiang Province, China)
出处
《微生物学免疫学进展》
2019年第5期10-15,共6页
Progress In Microbiology and Immunology
基金
“重大新药创制”科技重大专项(2015ZX09101044)
关键词
H7N9禽流感病毒
MF59佐剂
疫苗
免疫原性
血凝抑制抗体
微量中和抗体
Avian influenza A H7N9 virus
MF59 adjuvant
Vaccine
Immunogenicity
Hemagglutination inhibition antibody
Micro-neutralization antibody