“三棱-莪术”组分配伍介导PI3K-AKT通路治疗大鼠子宫肌瘤的研究

Study on PI3K-AKT Pathway Mediated by “Rhizoma Sparganii-Rhizoma Sparganii” Components in the Treatment of Rat Uterine Leiomyoma

目的:研究三棱-莪术对治疗子宫肌瘤的疗效及可能作用机制。方法:36只雌性SD大鼠随机分为空白组、模型组、1∶1组、1∶2组,每组9只,其中模型组、1∶1组、1∶2组制备子宫肌瘤模型,空白组、模型组用生理盐水灌胃,1∶1组、1∶2组根据三棱-莪术配伍比例进行灌胃,干预结束后比较各组子宫/卵巢系数、子宫大小,子宫病理形态以及PI3K/AKT通路标志因子的表达的变化。结果:1)模型组子宫平滑肌出现明显的病变,三棱-莪术干预后大鼠子宫组织病变有所改善,其中1∶1组改善较1∶2组明显。2)子宫肌瘤大鼠子宫系数、卵巢系数、子宫横竖径、子宫组织中E2和ER、PI3K、p-AKT/AKT表达水平均明显增加(P<0.05),经三棱-莪术干预后上述指标下降,其中术1∶1组改善较1∶2组明显(P<0.05)。结论:三棱-莪术配伍可改善子宫肌瘤,这一作用机制可能通过激活PI3K/AKT通路实现。

Objective: To study the therapeutic effect and possible mechanism of Rhizoma Sparganii-Rhizoma Sparganii in the treatment of uterine leiomyoma. Methods: A total of 36 female SD rats were randomly divided into a blank group,a model group,a 1∶ 1 group and a 1∶ 2 group,with 9 rats in each group.The model group,1∶ 1 group and 1∶ 2 group were used to prepare uterine myoma model.The blank group and the model group were given normal saline by gastric lavage.The 1∶ 1 group and 1∶ 2 group were given gastric lavage according to the compatibility ratio of trigonum and zedoary turmeric.After intervention,the changes of uterus/ovary coefficient,uterine size,uterine pathological morphology and the expression of PI3K/AKT pathway marker factors were compared in each group. Results: 1)The uterine smooth muscle in the model group showed obvious pathological changes,and the uterine tissue lesions improved after the intervention of Rhizoma Sparganii-Rhizoma Sparganii,and the improvement in the 1∶ 1 group was more obvious than that in the 1∶ 2 group.2)The uterine coefficient,ovarian coefficient,transverse and vertical diameter of uterus,and the expression levels of E 2,ER,PI3K and p-AKT/AKT in uterine tissue of rats with uterine leiomyoma significantly increased( P <0.05).The above indexes decreased after intervention with Rhizoma Sparganii-Rhizoma Sparganii.The improvement in 1∶ 1 group was more obvious than that in 1∶ 2 group( P <0.05). Conclusion: The compatibility of Rhizoma Sparganii-Rhizoma Sparganii can improve uterine leiomyoma,and this mechanism may be achieved by activating PI3K/AKT pathway.