基于网络药理学探讨银屑Ⅰ号治疗银屑病的作用机制

Analysis of Mechanism of Psoriasis Ⅰ Based on Network Pharmacology

目的应用网络药理学研究方法预测银屑Ⅰ号药物成分的作用靶点,并与银屑病相关靶点进行映射,拓扑出银屑Ⅰ号成分—靶点—疾病网络并进行富集分析,探讨银屑Ⅰ号治疗银屑病可能的作用机制。方法应用中药系统药理学(TCMSP)数据库和分析平台及文献检索挖掘出银屑Ⅰ号药物所对应的成分,通过中药成分靶点(HIT)数据库及Swiss靶点预测数据库检索出成分对应的靶点。在疾病靶点数据库(TTD),Drugbank(DBD)数据库及DisGeNet数据库中获取银屑病对应的靶点。使用Cytoscape构建银屑Ⅰ号成分—靶点—疾病网络及核心网络,应用ClueGo对靶点进行GO-BP富集分析及KEGG通路分析。结果筛选出银屑Ⅰ号成分—靶点—疾病网络中共42个相关靶点及59个相应化合物,共得出12条主要信号通路,其中血管内皮生长因子(VEGF)信号通路及皮质类固醇反应信号通路在网络中具有串话作用。结论银屑Ⅰ号通过直接或间接作用靶点调控多条信号通路,改善银屑病炎性浸润、角质形成细胞异常分化及异常新生血管生成等病理因素,从而发挥银屑病的治疗作用。

Objective To predict the targets of active components of Psoriasis Ⅰ(PⅠ) by the network pharmacology, to map related targets of psoriasis and analyze key nodes of interaction topologically, thus comprehensively exploring possible pharmacological mechanism of PⅠ for treating psoriasis. Methods The chemical ingredients of PⅠ were collected from Traditional Chinese Medicine Systems Pharmacology Database(TCMSP) and Analysis Platform and literature retrieval. Ingredients corresponding targets were obtained from Herbal Ingredients′ Targets(HIT) Database and Swiss Target Prediction Database(Swiss). Significant genes associated with psoriasis were retrieved from Therapeutic Target Database(TTD), Drugbank Database(DBD), and DisGeNet Database. The components-targets-psoriasis networks and core networks were constructed using Cytoscape. According to ClueGo, GO-BP enrichment analysis and KEGG pathway analysis of the targets were performed.Results Totally 59 correspondent components, 42 associated targets, and 12 main signal pathways were obtained. Of them, vascular endothelial growth factor(VEGF) signal pathway, and signal pathways response to corticosteroid were considered as bridge signal pathways with cross-talk functions. Conclusion PⅠ comprehensively improved pathological factors of psoriasis including inflammatory infiltration, abnormal differentiation of keratinocytes, and abnormal angiogenesis by regulating multiple signaling pathways directly or indirectly, thus playing therapeutic roles in treating psoriasis.