一株西沙海绵共附生真菌次生代谢产物

Secondary metabolites of sponge-derived fungus from Xisha

对采自西沙隋氏蒂壳海绵共附生真菌(Penicillium chrysogenum)次生代谢产物进行化学成分的研究,以期发现结构特异的次生代谢产物。隋氏蒂壳海绵共附生真菌用真菌2号培养基进行发酵,发酵后的菌体采用溶剂提取、萃取和现代色谱分离纯化手段,包括正相硅胶柱色谱、ODS柱色谱和HPLC分离制备,运用现代核磁波谱技术并结合高分辨质谱鉴定化合物的结构。结果显示:通过对隋氏蒂壳海绵共附生真菌的菌体提取物进行分离制备,最终分离得到7个单体化合物,鉴定结果为(1)Traumatic acid,(2)N-benzoyl-L-phenylalaninol,(3)cyclo-(Trp-Ser),(4)cyclo-(D-Pro-D-Leu),(5)cyclo-(Phe-Tyr),(6)(22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-(6),9(11),22-trien-3β-ol,(7)5α,8α-epidioxy-23-methyl-(22E,24S)-ergosta-6,22-dien-3β-ol。其中化合物1是从自然界中首次分离得到,化合物2、3、4、5、6、7是在该真菌内首次分离得到。

The secondary metabolites of strain Penicillium chrysogenum,an epiphytic fungus isolated from the Xisha sponge T.swinhoei were studied,in order to find structurally specific secondary metabolites.The fungus Penicillium chrysogenum was fermented with Fungus No.2 medium,the mycelium was extracted and purified by modern chromatography method including repeated column chromatography on silica gel,ODS and semi-preparative HPLC.The structures of isolated compounds were identified with modern spectroscopic techniques combined with HRMS.The results showed that seven compounds were isolated from the mycelium of strain Penicillium chrysogenum,which was isolated from the sponge T.swinhoei.They were identified as Traumatic acid(1),N-benzoyl-L-phenylalaninol(2),cyclo-(Trp-Ser)(3),cyclo-(D-Pro-D-Leu)(4),cyclo-(Phe-Tyr)(5),(22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol(6),5α,8α-epidioxy-23-methyl-(22E,24S)-ergosta-6,22-dien-3β-ol(7).Compound 1 was initially isolated from nature and compounds 2,3,4,5,6 and 7 were isolated from Penicillium chrysogenum for the first time.