外周血白细胞SREBPs表达水平作为冠心病诊断标志物的可行性研究

Feasibility Study of SREBPs Expression in Peripheral White Blood Cells as a Diagnostic Marker of Coronary Heart Disease

目的:探讨固醇调节元件结合蛋白(sterol regulatory elementbinding proteins,SREBPs)与冠心病的相关性。方法:选择冠心病患者65例及正常对照55例,利用RT-qPCR方法检测外周血白细胞SREBP1及SREBP2相对表达量;利用全自动生化分析仪检测血糖血脂等生化项目;使用受试者工作特征(receiver operating characteristic,ROC)曲线和曲线下面积(area under the curve,AUC)分析SREBPs对冠心病诊断的敏感度和特异度。结果:与对照组相比,冠心病组SREBP1及SREBP2相对表达量均显著下降,差异有统计学意义(P<0.001);SREBPs的ROC结果显示,SREBP1的AUC值为0.710,SREBP2的AUC值为0.697。结论:静脉血白细胞中SREBPs下调可能预示冠心病的发生。推测SREBPs可能成为冠心病早期诊断的一个潜在的生物标志物。

Objective To investigate the relationship between sterol regulatory element binding protein (SREBPs) and coro nary heart disease. Methods Relative expressions of REBP1 and SREBP2 in peripheral blood leukocytes were detected by RT-q PCR in 65 patients with coronary heart disease and 55 normal controls. Biochemical tests such as blood sugar and lip id were detected by automatic biochemical analyzer. Receiver operating characteristic ( ROC) curve and area under the curve ( AUC) were used to analyze the sensitivity and specificity of SREBPs in the diagnosis of coronary heart disease. Re sults Compared with the control group, the relative expression levels of SREBP1 and SREBP2 in the coronary heart disease group decreased significantly, and the difference was statistically significant (P< 0.001). The ROC curve analysis showed that SREBP1 AUC value was 0.710 and SREBP2 AUC value was 0.697. Conclusion Down-regulation of SREBPs in ve nous leukocytes may indicate the occurrence of coronary heart disease. It is speculated that SREBPs may be a potential bio marker for early diagnosis of coronary heart disease.