苜蓿胚性愈伤组织诱导及植株再生研究

Study on Induction of Embryogenic Callus and Plant Regeneration of Medicago sativa L.

以苜蓿5~7d苗龄无菌苗的子叶和下胚轴为外植体,研究培养基中不同激素和浓度配比对苜蓿愈伤组织诱导和分化的影响。结果表明,MS+2,4-D2.0mg/L+6-BA1.0mg/L愈伤组织诱导率达100%,且愈伤组织状态良好,增殖速度快。MS+6-BA0.5mg/L+NAA0.05mg/L为苜蓿胚性愈伤组织诱导的适宜培养基,诱导率为37.8%。胚性愈伤组织分化成苗培养基为MS+6-BA0.2mg/L+NAA0.01mg/L,生根培养基为1/2MS+NAA0.02mg/L+蔗糖10.0g/L。

Cotyledon and hypocotyls of 5~7 day's aseptic seedlings after germination were used as explants to study the effect of different hormones and concentrations on callus induction and differentiation with the combinative concentration of hormones. The results showed the callus induction medium was MS+2,4-D 2.0 mg/L+6-BA 1.0 mg/L. The ratio of callus was 100%. The callus grew well and proliferated fast. The embryogenic callus induction medium was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L. The medium for embryogenic callus differentiation into seedlings was MS+6-BA 0.2 mg/L+NAA 0.01 mg/L,and the rooting medium was 1/2MS+ NAA 0.02 mg/L+sucrose 10 g/L.