白藜芦醇抑制膀胱癌细胞体外侵袭能力的研究

Resveratrol inhibits the invasion capability of bladder cancer cells in vitro

目的探讨白藜芦醇对膀胱癌T24细胞体外迁移与侵袭能力的影响及可能的机制。方法采用划痕试验检测白藜芦醇对T24细胞体外迁移能力的影响,Transwell实验检测白藜芦醇对T24细胞体外侵袭能力的影响,Real-timePCR与Western检测白藜芦醇对MMP-2与MMP-9蛋白表达水平变化的影响。结果30μmol/L白藜芦醇处理T24细胞24h与48h后,划痕试验发现白藜芦醇可抑制T24细胞体外迁移能力,Transwell实验证实白藜芦醇可抑制膀胱癌T24细胞体外侵袭能力。Real-timePCR与Western结果表明,白藜芦醇可从mRNA与蛋白水平抑制MMP-2与MMP-9的表达,且呈时间依赖性。结论白藜芦醇可能通过下调MMP-2与MMP-9的表达而抑制膀胱癌T24细胞体外迁移与侵袭能力,可能成为治疗膀胱癌的新策略。

ObjectiveTo investigate the effects of resveratrol on the migration and invasion of bladder cancer T24 cells in vitro and the possible mechanism. MethodsThe effects of resveratrol on the migration and invasion of T24 cells were determined with wound healing test and Transwell assay,respectively.The effects of resveratrol on MMP-2 and MMP-9 expressions were detected with real-time PCR and Western blot. ResultsAfter resveratrol treatment for 24 h and 48 h,the migration and invasive capability of T24 cells were inhibited,and MMP-2 and MMP-9 expressions were decreased in a time-dependent manner. ConclusionResveratrol can inhibit the migration and invasion capability of T24 cells by downregulating the MMP-2 and MMP-9 expressions.Resveratrol may be a promising drug in the treatment of bladder cancer.