miR-320靶向调控FoxM1对结肠癌SW480细胞增殖、侵袭的影响

Effect of mi R-320 targeting FoxM1 on proliferation and invasion of colon cancer SW480 cells

目的分析miR-320对结肠癌SW480细胞增殖、侵袭的影响及其作用机制.方法将SW480细胞分为SW480组、mimic对照组、miR-320 mimic-/FoxM1 wt+/FoxM1 mut-组、miR-320 mimic+/FoxM1 wt +/FoxM1 mut-组、miR-320 mimic-/FoxM1 wt-/FoxM1 mut+组、miR-320 mimic+/FoxM1 wt-/FoxM1 mut+组、miR-320 mimic组、pc-FoxM1组、miR-320 mimic+pc-FoxM1组;检测各组细胞内凋亡、增殖、侵袭等相关指标.结果 miR-320 mimic细胞表达miR-320量显著升高(P<0.05),FoxM1表达量显著降低(P<0.05),荧光素酶报告实验结果显示,与FoxM1 wt组比较,FoxM1 wt+miR-320组荧光素酶活性显著降低(P<0.05);miR-320 mimic+pc-FoxM1组细胞细胞增殖数及PCNA蛋白表达水平均显著高于miR-320 mimic组(P<0.05);与miR-320 mimic组相比,miR-320 mimic+pc-FoxM1组细胞cleaved Caspase-9表达水平显著降低(P<0.05);与miR-320 mimic组相比,miR-320 mimic+pc-FoxM1组细胞迁移率显著升高(P<0.05);与miR-320 mimic组相比,miR-320 mimic+pc-FoxM1组细胞侵袭数显著升高(P<0.05).结论 miR-320通过靶向调控FoxM1实现抑制结肠癌SW480细胞增殖、侵袭能力.

Objective To analyze the effect of miR-320 on proliferation and invasion of colon cancer SW480 cells and its mechanism. Methods SW480 cells were divided into SW480 group, mimic control group, miR-320 mimic-/FoxM1 wt+/FoxM1 mut-group, miR-320 mimic+/FoxM1 wt +/FoxM1 mut-group, miR-320 mimic-/FoxM1 wt -/FoxM1 mut+ group, miR-320 mimic+/FoxM1 wt-/FoxM1 mut+group, miR-320 mimic group, pc-FoxM1 group, miR-320 mimic+pc-FoxM1 group. The proliferation, invasion and other related indicators between groups were compared. Results The expression of miR-320 in miR-320 mimic cells was significantly increased(P<0.05), and the expression of FoxM1 was significantly decreased(P<0.05). The results of luciferase reporter assay showed that the luciferase activity of FoxM1 wt+miR-320 was significantly decreased compared with that of the FoxM1 wt group(P<0.05). The cell proliferation and PCNA protein expression of miR-320 mimic+pc-FoxM1 group were significantly higher than that of miR-320 mimic group(P<0.05). Compared with that of the miR-320 mimic group, cell migration rate of miR-320 mimic+pc-FoxM1 group was significantly increased(P<0.05), and the expression level of cleaved Caspase-9 in the miR-320 mimic+pc-FoxM1 group was significantly lower(P<0.05). Compared with that of the miR-320 mimic group, the number of cell invasion was significantly increased in the miR-320 mimic+pc-FoxM1 group(P<0.05). Conclusion miR-320 can inhibit the proliferation and invasion of colon cancer SW480 cells by targeting FoxM1.