朱砂安神丸拮抗大鼠条件性恐惧及其睡眠障碍作用的研究

To Study the Impact of Zhusha’anshenwan to Rat with Conditioned Fear and Sleep Disorders

目的:通过实验研究,探究朱砂安神丸拮抗大鼠条件性恐惧及其睡眠障碍的作用。方法:首先进行大鼠条件性恐惧习得记忆、恐惧记忆消退的模型复制,观察条件性恐惧习得过程中、消退过程中大鼠的觉醒-睡眠时间及睡眠时相的变化,将雄性SD大鼠随机分为空白组、模型组及给药组。给药组实验动物给予朱砂安神丸混悬液(经灌胃给予,0. 81 g/kg)。空白组、模型组实验动物给予等体积的1%CMC,观察朱砂安神丸对大鼠条件性恐惧习得过程、恐惧记忆消退过程中觉醒-睡眠时间及睡眠时相的影响。结果:朱砂安神丸对大鼠条件性恐惧习得过程觉醒-睡眠时间及睡眠时相的研究发现,与模型组实验动物比较,给药组实验动物Wake时间缩短,百分比降低(P <0. 05)。睡眠总时间、REMS、NREM时间延长,百分比增加(P <0. 05或P <0. 01)。REMS与NREM的睡眠时间百分比无明显变化。朱砂安神丸对大鼠条件性恐惧记忆消退过程中觉醒-睡眠时间及睡眠时相的研究发现,与模型组比较,给药组实验动物的Wake时间缩短,百分比降低(P <0. 05)。睡眠总时间延长,百分比增加,NREM时间增加,第4天REM时间增加(P <0. 05或P <0. 01)。REMS与NREM的睡眠时间百分比无明显变化。结论:条件性恐惧能够引起觉醒时间延长,睡眠时间缩短而对REM及NREM的睡眠比例无影响;朱砂安神丸能够促进条件性恐惧消退;朱砂安神丸能够拮抗由条件性恐惧引起的睡眠障碍。

Objective: Through experimental study to investigate impact of zhusha′anshenwan to rat with conditioned fear and sleep disorders. Methods: Firstly to duplicate the model of conditioned fear habitual memory,extinction of conditioned fear memory of rat.To observe the changes of wake-sleep time and sleep phases during conditioned fear habitual process and extinction process.To randomized control group,model group and drug group.Drug group received zhusha′anshen suspension(intragastrical administration,0.81 g/kg).The control group and model group received the same volume of 1% CMC.To observe the impact of zhusha′anshen wan on wake-sleep time and sleep phases during conditioned fear habitual process and extinction process. Results: To observe the impact of zhusha′anshen wan on wake-sleep time and sleep phases during conditioned fear habitual memory of rat,comparing the model group,the wake time of drug group decreased and percentage decreased( P <0.05).Total sleep time,REMS and NREMS time increased and percentage increased( P <0.05 or P <0.01).The percentage of REMS and NREMS had no changes.To observe the impact of zhusha′anshen wan on wake-time and sleep phases during extinction of conditioned fear memory of rat,comparing model group,the wake time of drug group decreased and the percentage decreased( P <0.05).The total sleep time,the percentage,NREM time and the REM of forth day increased( P <0.05 or P <0.01).The percentage of REM and NREM had no changes. Conclusion: Conditioned fear can increase wake time and decrease sleep time.It has no impact on the percentage of REM and NREM.Zhusha′anshen wan can prompt extinction of conditioned fear.It can counter the sleep disorders caused by conditioned fear.