慢阻肺患者血清中组蛋白去乙酰化酶2 IL-1βIL-6及IL-17A的水平及临床意义

Serum Levels of Histone Deacetylase 2 Interleukin-1 beta Interleukin-6 and Interleukin-17A in Patients with COPD and Clinical Significance

目的:探究慢阻肺患者血清中组蛋白去乙酰化酶2、白介素-1β、白介素-6及白介素17A的水平及其临床意义。方法:回顾性选取2016年6月至2018年6月我院呼吸重症监护室64例稳定期重度慢阻肺患者作为观察组,64例体检健康的正常者作为对照组。ELISA测定血清HDAC2、IL-1β、IL-6和IL-17A水平,肺功能检测仪检测FEV1/FVC%和FEV1%pred,Pearson相关分析探究血清HDAC2、IL-1β、IL-6和IL-17A水平与肺功能的相关性。结果:与对照组健康人群比较,观察组慢阻肺患者血清HDAC2表达明显降低,IL-1β、IL-6和IL-17A水平则显著升高(P<0.05)。慢阻肺患者随着肺功能分级的增加,血清HDAC2含量逐渐降低,而IL-1β、IL-6和IL-17A水平逐渐升高(P<0.05)。不同级别肺功能的慢阻肺患者FEV1/FVC%和FEV1%pred随着分级的增加而逐渐降低,比较结果有统计学差异(P<0.05)。Pearson相关分析结果显示,慢阻肺患者血清HDAC2与肺动能正相关,IL-1β、IL-6、IL-17A水平与肺动能呈显著负相关(P<0.05)。结论:稳定期慢阻肺患者血清HDAC2表达下调,而炎性因子IL-1β、IL-6、IL-17A水平则均升高。患者气道、肺泡组织炎症反应激活可能是肺功能进行性下降的主要因素之一。

Objective: To explore the plasma histone acetylation enzyme 2,interleukin 1,interleukin 6 and interleukin 17A levles of patients with COPD and the clinical significance. Methods: 64 COPD patients were retrospectively chosen as the observation group in our respiratory intensive care unit from June 2016 to June 2018. While,64 cases of normal physical health people were selected as the control group. Serum HDAC2,IL-1β,IL-6 and IL-17A levels were determined by ELISA. FEV1/FVC% and FEV1% pred values were detected by the lung function detector. Pearson correlation analysis was performed to explore the correlation between serum HDAC2,IL-1β,IL-6 and IL-17A levels and lung function. Results: Compared with the control group,serum HDAC2,IL-1β,IL-6 and IL-17A levels were significantly increased in the observation group (P<0.05). Serum HDAC2,IL-1β,IL-6 and IL-17A levels of observation group were gradually increased with the lung function grade increase (P<0.05). FEV1/FVC%,FEV1% pred of COPD patients with different levels of lung function decreased gradually with the increase of grade,and the comparison difference results were statistically significant (P<0.05). Pearson correlation analysis showed that serum HDAC2 level was positively correlated with lung function. While,IL-1β,IL-6 and IL-17A levels were negatively correlated with lung function FEV1/FVC% and FEV1% pred (P<0.05). Conclusion: The expression of histone deacetylase 2 was decreased,IL-1β,IL-6 and IL-17A levels of COPD patients were significant increased. The inflammation of patient airway and alveolar tissue were activated,which might be one of the main factors of progressive decline of lung function.