妇科再造丸含药血清对人子宫肌瘤细胞Bax和Bcl-2蛋白表达的影响

Effect of Fukezaizao Pill Medicated Serum on the Expression of Bax and Bcl-2 Proteins in Human Uterine Fibroid Cells

目的:探讨妇科再造丸(FKW)含药血清对体外培养人子宫肌瘤细胞凋亡相关蛋白Bax和B细胞淋巴瘤-2(Bcl-2)表达的影响。方法:对人子宫肌瘤细胞进行原代培养、传代并进行鉴定;60只SD雌性大鼠随机均分为空白组(无药血清组),FKW高、中、低剂量组和桂枝茯苓胶囊(GZFL)组,利用各组大鼠制备无药血清和含药血清分别作用于子宫肌瘤细胞72h时,采用WesternBlot检测细胞凋亡相关蛋白Bcl-2、Bax的表达。结果:培养的细胞经鉴定证实为人子宫肌瘤细胞;与无药血清组比较,FKW含药血清各剂量组均能增加Bax蛋白相对表达量,降低Bcl-2蛋白相对表达量(P<0.05或P<0.01),且作用随剂量的增加而有增强的趋势(P<0.05或P<0.01)。结论:FKW含药血清可影响人子宫肌瘤细胞凋亡相关蛋白Bcl-2、Bax的表达,这可能是FKW临床应用时缓解子宫肌瘤症状的机制之一。

Objective:To investigate the effect of Fukezaizao pill(FKW)medicated serum on the expression of Bax and Bcl-2 in human uterine fibroid cells in vitro.Methods:Primary culture,passage and identification of human uterine fibroid cells were carried out.Sixty SD female rats were randomly divided into blank group(drug-free serum group),FKW high,medium and low dose groups and Guizhifulin capsule(GZFL)group.When the drug-free serum and drug-containing serum of each group of rats acted on uterine fibroid cells for 72 h respectively,western-blot was used to detect the expression of apoptosis related protein Bcl-2 and Bax.Results:The cultured cells were identified as human uterine fibroid cells.Compared with the non-drug serum group,the relative expression of Bax protein increased and the relative expression of Bcl-2 protein decreased in each dose group of FKW containing serum(P<0.05 or P<0.01),and the effect was enhanced with the increase of dose(P<0.05 or P<0.01).Conclusion:FKW containing serum can affect the expression of apoptosis related protein Bcl-2 and Bax in human uterine fibroid cells,which may be one of the mechanisms of FKW to relieve the symptoms of uterine fibroid.