香鳞毛蕨化学成分及其体外抗浅部真菌活性研究

Study on chemical constituents and antifungal activities in vitro of Dryopteris fragrans (L.) Schott

为对香鳞毛蕨的化学成分进行提取分离及体外抗浅部真菌活性研究。本实验采用硅胶柱色谱、Sephadex LH-20凝胶以及制备液相色谱方法对香鳞毛蕨的化学成分进行分离纯化,得到8个化合物,根据理化性质和波谱数据分析确定所得化合物的结构,分别鉴定为白绵马素PB(1)、绵马素PP(2)、黄绵马酸BB(3)、田基黄绵马酸A(4)、filicinic acid Ⅵ(5)、mallophenone(6)、绵马素AB(7)、绵马酚B(8),其中化合物1~6均为首次从该植物中分离得到,经抗真菌筛选,发现化合物3~8对红色毛癣菌和石膏样小孢子菌具有较好的抑制作用,可为进一步开发其药用价值奠定基础。

This paper studied the chemical constituents and antifungal activities in vitro of Dryopteris fragrans(L.)Schott.Eight compounds were isolated from Dryopteris fragrans(L.)Schott by column chromatography on silica gel,Sephadex LH-20,Semi-prepared liquid phase and identified by physico-chemical properties and spectroscopic analysis.Their structures were elucidated as albaspidin PB(1),aspidin PP(2),flavaspidic acid BB(3),saroaspidin A(4),filicinic acid Ⅵ(5),mallophenone(6),aspidin AB(7),aspidinol B(8),among which compounds 1-6 were obtained from this plant for the first time.After antifungal test,compounds 3-8 have strong inhibitory effects on Trichophyton rubrum and Microsporum gypseum.It can lay a foundation for further development of Dryopteris fragrans(L.)Schott medicinal value.