土壤链霉菌KIB-K1次级代谢产物中大环内酯及其抗菌活性研究

Macrolides from soil-derived Streptomyces sp. KIB-K1 and their antimicrobial activities

为研究土壤链霉菌Streptomyces sp.KIB-K1次级代谢产物成分。实验采用硅胶、凝胶及半制备高效液相等分离方法,从该菌株发酵液中共分离得到7个大环内酯类化合物,通过解析NMR、MS等波谱数据,化合物的结构鉴定为aldgamycin P(1)、aldgamycin E(2)、aldgamycin J(3)、aldgamycin K(4)、aldgamycin L(5)、GERI-155(6)和chalcomycin(7),其中化合物1为新化合物。抗菌实验结果显示,化合物1~7对金黄色葡萄球菌和枯草芽孢杆菌有显著的抑制作用。其中,化合物1对金黄色葡萄球菌的最小抑菌浓度(MIC)为7.81μg/mL,化合物2和7对金黄色葡萄球菌的MIC值分别为0.49和0.98μg/mL,高于阳性对照卡那霉素的MIC值(3.91μg/mL)。化合物1~7对于被测试的两株真菌小麦赤霉病菌和玉米小斑病菌没有表现出抑制作用。

The secondary metabolites of soil Streptomyces sp.KIB-K1 were investigated.Seven macrolides were isolated by the means of various chromatography methods,such as silica gel column,Sephadex LH-20 column and pre-HPLC,from the fermentation broth of Streptomyces sp.KIB-K1.Their structures were identified as aldgamycin E(2),aldgamycin J(3),aldgamycin K(4),aldgamycin L(5),GERI-155(6),chalcomycin(7)and a new compound,aldgamycin P(1),by analyzing the NMR and Mass data.The result of antibacterial test showed that these macrolides exhibited significant inhibitory effect against Staphylococcus aureus ATCC 6538 and Bacillus subtilis ATCC 6633.The minimal inhibit concentration(MIC)of aldgamycin P(1)to S.aureus was 7.81μg/mL.The MIC of compounds 2 and 7 to S.aureus were 0.49 and 0.98μg/mL,respectively,which was higher than that of positive control kanamycin with MIC of 3.91μg/mL.However,compounds 1-7 showed no inhibitory effect against two pathomycetes of plant,Gibberella zeae and Helminthosporium maydis Nisik&Miy.