真菌NBERC49006的次级代谢产物及其除草活性研究

Secondary metabolites from fungus NBERC49006 with herbicidal activity

为明确除草活性真菌NBERC_49006中的活性物质基础,对其次级代谢产物进行研究。通过对菌株大量发酵,采用乙酸乙酯提取,凝胶柱色谱和制备高效液相色谱分离纯化得到7个化合物,并通过理化性质、LC-MS及NMR鉴定为trichothecin(1)、8-deoxy-trichothecin(2)、engleromycenolic acid a(3)、engleromycenolic acid b(4)、rosololactone(5)、rosenonolactone(6)、2α-hydroxy-cis-communic acid(7)。其中,化合物3、4和7首次从该菌种中得到,而且化合物7是首次从微生物中得到。除草活性测试表明化合物1和2对拟南芥具有较强的除草活性,LC 50值分别为9.30和28.98μg/mL。该研究可为真菌源除草先导化合物的发现提供良好的研究基础。

In order to clarify the active substance basis of herbicidal fungus NBERC_49006,its secondary metabolites were studied.Seven compounds were isolated by large-scale fermentation,extraction,preparative HPLC and gel column chromatography.The structures were elucidated on the basis of spectral characteristics and identified as:trichothecin(1),8-deoxy-trichothecin(2),engleromycenolic acid A(3),engleromycenolic acid B(4),rosololactone(5),rosenonolactone(6),2α-hydroxy-cis-communic acid(7).Among them,compounds 2,4 and 7 were isolated from the strain for the first time,and compound 7 was obtained from microorganism for the first time.The results of bioassay test showed that compounds 1 and 2 had obvious herbicidal activity against Arabidopsis thaliana with the LC 50 value of 9.30 and 28.98μg/mL,respectively.The results of this study can provide a good basis for the discovery of lead compounds with herbicidal activities from fungal.