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枇杷叶提取物中科罗索酸在糖尿病大鼠体内的药动学研究

Plasma concentration of corosolic acid in loquat leaf extract in diabetic rats by HPLC and its pharmacokinetics study
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摘要 〔目的〕建立高效液相色谱法(high performance liquid chromatography,HPLC),测定枇杷叶提取物中科罗索酸在糖尿病大鼠体内的血药浓度,并探讨其在病理模型大鼠体内药动学特征。〔方法〕SD糖尿病模型大鼠给予枇杷叶提取物灌胃,在不同时间点眼后静脉丛取血,预处理后测定血浆中科罗索酸的浓度,经DAS 2.0软件处理数据。〔结果〕枇杷叶提取物中科罗索酸在糖尿病大鼠体内的药动学符合二房室模型,科罗索酸在体内较快地被吸收,给药后1.5 h即达到峰浓度,峰浓度为(0.922±0.182)mg/L,其他主要药动学参数:吸收速率常数为(2.754±0.382)/h、分布半衰期为(0.879±0.295)h、消除半衰期为(8.752±3.561)h、24 h药时曲线下面积为(5.528±1.357)mg·h/L。〔结论〕高效液相色谱法测定血浆中科罗索酸浓度准确、简便,适用于枇杷叶提取物中科罗索酸的药动学研究。 〔Objective〕To establish a high performance liquid chromatography ( HPLC) method for measuring the concentration of corosolic acid in loquat leaf extract in diabetic rats and to study its pharmacokinetics.〔Methods〕Blood sample were collected through venous plexus in rat eye at different time after oral administration of loquat leaf extract. After liquide extraction with acetonitrile, the concentration of corosolic acid in plasma was measured. Then pharmacokinetics parameters were calculated with DAS 2. 0 program.〔Results〕The results showed that corosolic acid plasma concentration-time curves in diabetic rats coincided with two-compartment model, corosolic acid is absorbed fast in rats. Peak concentration of corosolic acid in rat plasma is (0. 922 ±0. 182) mg/L at 1. 5 hour. The main pharmacokinetic parameters: absorption rate constant is ( 2. 754 + 0. 382)/h, distribution half time is (3. 979 ±1. 295) h, elimination half time is (8. 752 ±3. 561) h, area under the concentration-versustime curve is ( 5. 528 ± 1. 357 ) mg· h/L.〔Conclusion〕HPLC method is accurate and sensitive, it is suitable for the pharmacokinetic study of corosolic acid in loquat leaf extract in diabetic rats.
作者 李海岛 曹旭 LI Haidao;CAO Xu(Jiangsu Jibrier Pharmaceutical Joint Stock Co. Ltd. , Zhenjiang 212009, China)
出处 《河南大学学报(医学版)》 CAS 2019年第3期166-169,共4页 Journal of Henan University:Medical Science
关键词 科罗索酸 枇杷叶提取物 药动学 高效液相色谱法 corosolic acid loquat leaf extract pharmacokinetics HPLC
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