超低频电磁场处理水对氧嗪酸钾致大鼠高尿酸血症的调节作用

Regulating effects of extremely-low frequency electromagnetic field-treated water on hyperuricemia induced by oteracil potassium in rats

目的探讨饮用超低频电磁场处理水(简称频谱水)对氧嗪酸钾诱致大鼠高尿酸血症的改善作用。方法将56只SPF级SD雄性大鼠随机分为2组,即正常对照组(14只)和染毒组(42只)。染毒组大鼠每天灌胃2 g/kg氧嗪酸钾悬浮液,灌胃容量为10 ml/kg,正常对照组给予等容积的0.5%羧甲基纤维素钠,每天1次,连续7 d。将染毒组大鼠随机分为高尿酸血症对照组、频谱水拮抗组、别嘌醇拮抗组,每组14只。然后,三组大鼠继续给予氧嗪酸钾(2 g/kg)染毒,高尿酸血症对照组每天饮用普通水,频谱水拮抗组每天饮用频谱水,别嘌醇拮抗组灌胃给予别嘌醇(25 mg/kg),共60天。于受试物处理第30天、第45天时眼静脉取血测定血清尿酸水平,第60天时于末次处理50min后,3%戊巴比妥钠(30 mg/kg)腹腔注射,麻醉后腹主动脉取血,测定血清尿酸水平及血清尿素氮和肌酐水平;同时,采用ELISA法测定白介素1β(IL-1β)和肿瘤坏死因子(TNF-α)的水平。结果高尿酸血症对照组大鼠的血清尿酸水平高于正常对照组,差异均有统计学意义(P<0.05);第30、45、60天时频谱水拮抗组和别嘌醇拮抗组大鼠的血清尿酸水平低于高尿酸血症对照组,差异均有统计学意义(P<0.05)。高尿酸血症对照组大鼠血清肌酐水平略高于正常大鼠,但差异无统计学意义(P>0.05);而血尿素氮水平高于正常对照组,差异有统计学意义(P<0.05)。与高尿酸血症对照组比较,频谱水拮抗组大鼠血清肌酐和尿素氮水平略有降低,但差异均无统计学意义(P>0.05);而别嘌醇拮抗组大鼠血清尿素氮水平增加,差异有统计学意义(P<0.05)。高尿酸血症对照组大鼠血清IL-1β和TNF-α水平均高于正常对照组,差异有统计学意义(P<0.05);频谱水拮抗组和别嘌醇拮抗组大鼠血清IL-1β和TNF-α的水平均低于高尿酸血症对照组,差异有统计学意义(P<0.05)。结论饮用频谱水对氧嗪酸钾诱致高尿酸血症大鼠具有降低血清尿酸水平的作用,对高尿酸引起的肾脏病理性改变具有一定的保护作用,其作用机制可能与降低IL-1β和TNF-α水平有关。

Objective To understand the regulating effects of the extremely-low frequency electromagnetic field-treated water (spectrum water) on the hyperuricemia induced by oteracil potassium (OP) in rats. Methods A total of 56 SPF grade male SD rats were randomly divided into two groups, normal control group (14 rats) and oteracil potassium (OP) exposure group (42 rats). Rats of OP exposure group were treated with oteracil potassium suspension through gavage at dose of 2g/kg with volume of 10 ml/kg and normal control group was given 0.5% carboxymethylcellulose sodium with the same volume of oteracil potassium suspension, once a day for seven consecutive days.Then the rats of OP exposure group were randomly divided into three groups, including hyperuricemia control group, spectrum water antagonist group and allopurinol antagonist group, 14 rats in each group,rats of which were continuously treated with oteracil potassium (2g/kg ), and the rats in hyperuricemia control group drank ordinary water,in spectrum water antagonist group drank spectrum water every day,in allopurinol antagonist group were treated by allopurinol (25 mg/kg) for 60 days. The blood uric acid come from vena ophthalmica was measured at the 30th and 45th days of treatment.At the end of the 60-day treatment (50 min after the last treatment),the abdominal aorta blood was sampled after an intraperitoneal injection of 3% sodium pentobarbital (30 mg/kg) for all rats,and the serum uric acid,urea nitrogen and creatinine were determined. At the same time,ELISA was used to determine the levels of IL-lβ and TNF-α. The kidneys were taken for HE staining and the changes of kidney tissue were observed under light microscope. Results The serum uric acid levels of rats in hyperuricemia control group were significantly higher then those in control group (P<0.05),and in spectrum water antagonist group and allopurinol antagonist group at the 30th,45th and 60th day of treatment (P<0.05). The rats in hyperuricemia control group showed higher levels of serum creatinine (P>0.05) and urea nitrogen (P<0.05) compared with those in control group. Compared with hyperuricemia control group, lower levels of serum creatinine and urea nitrogen were observed in spectrum water antagonist group (P>0.05), higher levels of serum urea nitrogen were observed in allopurinol antagonist group ( P <0.05).The levels of IL-lβ and TNF-α in serum of the rats in hyperuricemia control group were significantly higher higher compared with those in control group, spectrum water antagonist group and allopurinol antagonist group (P<0.05). Conclusion Drinking spectrum water has a significant reduction in serum uric acid levels in hyperuricemia rats induced by oteracil potassium. It has a certain protective effect on the renal pathological changes caused by hyperuricemia, and its mechanism may be related to the reduction of IL-lβ and TNF-α levels.