龙眼HD-Zip基因家族生物信息及表达分析

Biological Information and Expression Analysis of HD-Zip Gene Family in Dimocarpus longan Lour.

为了解龙眼HD-Zip基因家族的功能,本研究基于龙眼基因组与转录组数据库,对提取的19个龙眼HD-Zip家族成员的启动子、可变剪接以及受到调控的miRNA种类进行分析;并采用实时荧光定量PCR技术,分析了在ABA处理下龙眼胚性愈伤组织(embryonic callus,EC)中HD-Zip部分成员的表达模式,以及HD-Zip部分成员在不同体胚阶段的表达模式。结果表明:龙眼HD-Zip家族除含有TATA-box和CAAT-box以外还含有大量的光响应元件、激素响应元件以及胁迫响应元件,暗示龙眼HD-Zip家族成员可能参与光反应、激素响应以及非生物胁迫的过程;龙眼HD-Zip在不同组织部位与不同体胚阶段的可变剪接方式主要以内含子保留为主;龙眼HD-Zip基因家族成员主要受到miRNA166a的调控;在外源激素ABA处理下龙眼EC中HD-Zip家族部分成员的表达模式以及部分成员在不同体胚阶段的表达模式都呈现多样化,推测龙眼HD-Zip参与龙眼不同体胚发育的调控,并且可能通过调节内源ABA的浓度进而影响胚性愈伤组织的生长发育以及形态的建成。

In order to understand the function of the longan HD-Zip gene family, this study was based on the longan genome and transcriptome database, and analyzed the promoters, alternative splicing and regulated miRNA species of 19 longan HD-Zip family members, and the expression pattern of HD-Zip members in longan embryogenic callus under ABA treatment and the expression patterns of some members at different somatic stages was analyzed by real-time fluorescent quantitative PCR. The longan HD-Zip family contained a large number of photoresponsive elements, hormone response elements and stress response elements in addition to TATA-box and CAAT-box, suggesting that longan HD-Zip family members may be involved in photoreactivity, hormone response and non-biological stress. The alternative splicing mode of longan HD-Zip in different tissue parts and different somatic embryo stages was mainly intron retention. The longan HD-Zip gene family members were mainly regulated by miRNA166a. The expression patterns of some members of the HD-Zip family in the longan EC stage under the treatment of exogenous hormone ABA and the expression patterns of some members in different somatic embryo stages were diverse. It is speculated that longan HD-Zip participates in the regulation of different somatic embryo development in longan, and may affect the growth and development of embryogenic callus and the formation of morphology by regulating the concentration of endogenous ABA.