沙棘浆果中酚类物质含量与自由基清除能力相关分析

Correlation Analysis of Phenolic Compound Content and Hydroxyl Radical Scavenging Capacity in Seabuckthorn Berry

[目的]本研究分析沙棘(Hippophae rhamnoides L.)果实中芦丁、槲皮素、异鼠李素、山奈酚、杨梅素、柚皮素和维生素E这7种酚类物质与羟自由基清除能力的关系,为探讨酚类物质对羟自由基清除能力提供参考。[方法]以沙棘丰宁和向阳2栽培品种3个时期(绿果期、变色期和成熟期)的果实为实验材料,利用气相色谱-质谱联用(GC-MS)技术测定芦丁、槲皮素、异鼠李素、山奈酚、杨梅素、柚皮素和维生素E这7种酚类物质含量,同时利用分光光度计法测定沙棘果的羟自由基清除能力,随后将7种酚类物质与羟自由基清除能力进行相关性分析。[结果]表明:丰宁羟自由基清除能力显著高于向阳,且羟自由基清除能力在绿果期、变色期和成熟期依次降低,而向阳在绿果期羟自由基清除能力最强,变色期最弱;丰宁果肉中芦丁、槲皮素、异鼠李素、山奈酚、杨梅素和柚皮素6种酚类物质含量高于向阳,其中,芦丁和杨梅素含量在3个不同时期均显著高于向阳(P<0.05);另外,果实3个不同发育时期中槲皮素、异鼠李素、柚皮素和杨梅素这4种物质含量在2沙棘品种中均表现出先上升后下降的趋势,而芦丁、维生素E和山奈酚这3种物质在2沙棘品种中变化趋势不同。相关性分析发现:芦丁、槲皮素、异鼠李素、山奈酚、杨梅素和柚皮素与羟自由基清除能力的相关性系数均大于0.6,而维生素E与羟自由基清除能力相关性系数仅为0.17。[结论]芦丁、槲皮素、异鼠李素、山奈酚、杨梅素和柚皮素的含量与羟基自由基的清除具有正相关性,有助于促进沙棘果实中羟自由基的清除。

[Objective] The relationship between hydroxyl radical scavenging capacity and seven phenolic substances in Seabuckthorn fruits was analyzed to provide reference for exploring the hydroxyl radical scavenging capacity of phenolic substances.[Method] The sea buckthorn fruits of Fengning cultivar and Xiangyang cultivar in three development stages (green fruit stage, discoloration stage and mature stage) were used as experimental materials.The contents of seven phenolic substances such as rutin, quercetin, isorhamnetin, kaempferol, myricetin, naringenin and vitamin E were determined by gas chromatography-mass spectrometry (GC-MS) technique.The hydroxyl radical scavenging ability of sea buckthorn fruit was determined by spectrophotometry.Then the correlation between the seven phenolic substances and hydroxyl radical scavenging capacity was analyzed.[Result] The results showed that the hydroxyl radical scavenging ability of Fengning cultivar was significantly higher than that of Xiangyang cultivar, the hydroxyl radical scavenging ability of Fengning cultivar decreased successively in the three development stages, while that of Fengning cultivar was the strongest in green fruit stage and the weakest in discoloration stage.The contents of rutin, quercetin, isorhamnetin, kaempferol, myricetin and naringenin in the sarcocarp of Fengning cultivar were higher than those in Xiangyang cultivar.The contents of rutin and myricetin in the sarcocarp of Fengning cultivar were significantly higher than those in Xiangyang cultivar during the three stages ( P <0.05).In addition, the contents of quercetin, isorhamnetin, naringenin and myricetin increased at first and then decreased in the three developmental stages, while the changes of rutin, vitamin E and kaempferol were different between the two cultivars.The correlation analysis showed that the correlation coefficients between rutin, quercetin, isorhamnetin, kaempferol, myricetin and naringenin and hydroxyl radical scavenging ability were all greater than 0.6, while the correlation coefficients between vitamin E and hydroxyl radical scavenging ability were only 0.17.[Conclusion] The hydroxyl radical scavenging ability are strongly correlated with the contents of rutin, quercetin, isorhamnetin, kaempferol, myricetin and naringenin, which were helpful to promote the scavenging of hydroxyl radicals in seabuckthorn fruit.