一种基于N基因的麻疹病毒实时荧光RT-PCR检测技术

Study on the N gene-based real-time RT-PCR assay for the detection of measles viru

目的引进一种以检测麻疹病毒N基因为目标的荧光RT-PCR技术并运用到新余市麻疹常规监测工作。方法新荧光RT-PCR与国产的荧光RT-PCR试剂盒、IgM抗体ELISA检测试剂盒分别对新余市2014年所采集麻疹疑似病例咽拭子、血清样本进行检测和结果分析。结果新荧光RT-PCR、国产的荧光RT-PCR试剂盒、ELISA试剂盒的检测阳性率分别为25.53%、23.4%、17.02%。与ELISA相比,RT-PCR具有更好的检测灵敏度,且两种荧光RT-PCR之间的总符合率达到97.87%。结论新引进的方法很敏感,适用于麻疹实验室监测以及疑似病例的快速诊断。

Objective To introduce a real-time RT-PCR assay targeting the N gene of measles virus and to apply it in routine surveillance of measles in Xinyu. Methods Collected from measles-suspect cases in Xinyu during the whole 2014,the throat swab specimens were detected for viral RNA by this new technique and some domestic real-time RT-PCR kit respectively,and the serum specimens were tested by ELISA kit for IgM antibody,then the data obtained were analyzed. Results The positive ratios of this new RT-PCR,the domestic RT-PCR kit and the ELISA kit were 25.53%,23.4% and 17.02% respectively. Compared to the ELISA,the two real-time RT-PCR methods had better sensitivity and the total compliance of them was 97.87%. Conclusion This new real-time RT-PCR technique is very sensitive to detect measles virus,and it is suitable for laboratory surveillance of measles and rapid diagnosis of measles-suspect cases.