人工老化对沙葱种子中活性氧与超微弱发光的影响

Effects of Artificial Aging on ROS and UWL in the Seeds of Allium mongolicum

以人工老化和Ce^3+浸种后再人工老化的沙葱种子为试材,对种子活力、电导率、呼吸速率、活性氧和超微弱发光等指标进行了研究,探讨Ce3+浸种对沙葱老化种子活性氧与超微弱发光的影响。结果表明,Ce^3+1处理下,沙葱种子的发芽率、活力指数和胚根长分别比ck1高4%、0.042和1.64cm,表明Ce^3+有助于提高种子活力及促进胚根的伸长;在老化程度较低的情况下,Ce^3+前处理对沙葱种子电解质外渗有一定缓解作用;活性氧在老化15h前,Ce^3+处理的超氧阴离子产生速率低于ck;Ce^3+比ck的超微弱发光强度高,Ce^3+1和Ce^3+0的超微弱发光强度差异不显著,Ce^3+0显著高于Ce^3+2、Ce^3+3和Ce^3+4的超微弱发光强度。超微弱发光与胚根长、超氧阴离子产生速率和过氧化氢含量呈极显著正相关,表明超微弱发光强度随活性氧的变化而变化。在种子的加速老化过程中,Ce3+处理可调控活性氧的增加,减缓老化对细胞损伤,维持细胞功能稳定性。

In order to explore the effects of cerium (Ce ^3+)-soaking seed on physiological indicators including reactive oxygen and ultra-weak light emission,we detected the seed activity,electrical conductivity,respiration rate,reactive oxygen and ultra-weak light emission of Allium mongolicum seeds treated with artificial aging and artificial aging after cerium (Ce^ 3+)-soaking seeds respectively.The results showed that the germination rate,vigor index and radicle length of A. mongolicum seeds under Ce ^3+ 1 treatment were 4%,0.042 and 1.64 cm higher than ck 1,indicating that Ce 3+ could improve seed vigor and promote radicle elongation.Under the condition of low aging degree,pre-treatment of Ce ^3+ could alleviate electrolyte exosmosis of shallot seeds to some extent.Additionally,Before aging 15 hours,production rate of the superoxide anion in Ce ^3+ treatment seeds was lower than that of ck,while ultra-weak luminescence intensity in Ce ^3+ treatment seeds was higher than that of ck.There was no significant difference in the ultra-weak luminescence intensity between Ce ^3+ 1 and Ce ^3+ 0.And the ultra-weak luminescence intensity of Ce^ 3+ 0 treatment seeds was significantly higher than that in Ce^ 3+ 2,Ce ^3+ 3 and Ce^3+ 4.The ultra-weak luminescence intensity was significantly positively correlated with radicle length,anion production rate and hydrogen peroxide content,indicating that the ultra-weak luminescence intensity changed with the change of reactive oxygen species.In a word,in the accelerated aging process of seeds,Ce^3+ treatment could regulate the increase of reactive oxygen species,slow down the damage of aging to cells,and maintain the stability of cell function.