黑曲霉作为分泌蛋白细胞工厂的研究进展

Progress in Aspergillus niger as cell factory for secretory proteins

黑曲霉具有极强的分泌蛋白的能力,其分泌的木质纤维素降解酶、淀粉酶、蛋白酶、脂肪酶等广泛应用于食品、饲料和生物技术等相关工业中。本文围绕黑曲霉生产同源和异源分泌蛋白,阐述黑曲霉作为分泌蛋白细胞工厂的巨大潜力。首先,通过总结黑曲霉表达系统的优越性,确定了黑曲霉作为分泌蛋白细胞工厂的开发前景。随后,介绍了初步构建黑曲霉分泌蛋白细胞工厂的一般流程。接着,总结了近十年来黑曲霉作为同源分泌酶细胞工厂的研究进展,提出在黑曲霉中定向表达分泌酶是深入研究黑曲霉自身分泌酶性质、功能和结构的理想策略。最后,总结了近年来黑曲霉作为异源分泌蛋白细胞工厂的研究进展,并从异源蛋白表达中遇到的难点出发介绍了完善黑曲霉细胞工厂来提高异源蛋白产量的对策。

Aspergillus niger has a strong ability to secrete proteins,and its secreted lignocellulose degrading enzymes,amylases,proteases,and lipases are widely used in related industries such as food,feed,and biotechnology.We describe its great potential as cell factory for secretory proteins based on the production of homologous and heterologous secretory proteins by A.niger.Firstly,the prospect of A.niger as cell factory for secretory proteins is ascertained based on the superiority of its expression system.Then the standard procedure for construction of A.niger cell factory for secretory proteins is introduced.Subsequently,the advances on A.niger as cell factory for homologous secretases in the past decade are summarized.It is proposed that the directed expression of inherent secretases in A.niger is an ideal strategy to study their properties,functions and structures.Finally,the advances on A.niger as cell factory for heterologous secretory proteins in recent years are summarized,and based on the difficulties encountered in the expression of heterologous proteins,the countermeasures to improve the production of heterologous proteins by further improvement of A.niger cell factory are introduced.