美人蕉(Canna indica)内生细菌促生能力及其强化水体的净化作用

Growth promoting ability and enhanced effects on water purification of endophytic bacteria from Canna indica

从美人蕉(Canna indica)植物体内分离内生细菌.通过测定内生细菌产吲哚乙酸(IAA)、铁载体、固氮、溶磷能力筛选出3株内生细菌C01、C16、C20.本试验的目的为评价接种内生细菌能否促进美人蕉种子萌发和幼苗生长,并通过小型浮床水质净化试验,评价接种内生细菌后的美人蕉对水体中营养物质的去除效果.结果表明:C01、C16、C20促进了美人蕉种子的萌发、生长,C16试验组萌发率为83.3%,显著高于对照组31.1%;C16显著提高了植株的株高和根长,分别比对照组高出23.33%、40.44%.水质净化实验阶段,C16试验组总磷、总氮的去除率分别达到88.4%、96.9%,分别比对照组高出26.4%、15.6%.最终,C01、C16试验组生物量、根长都显著高于对照组.C16试验组植株体内可溶性糖含量、可溶性蛋白显著高于对照组317.3%、97.8%.经鉴定,C01为荧光假单胞菌(Pseudomonas fluorescens),C16为肠杆菌(Enterobacter sp.),C20为欧文氏菌(Erwinia sp.).

Endophytic bacteria were isolated from Canna indica, and three strains C01, C16 and C20 were screened out regard to their abilities of producing indoleacetic acid (IAA) and siderophore, nitrogen fixation, as well as phosphate solubilization. The aim of this study is to evaluate the influence of inoculating of endophytic bacteria on seed germination and seedling growth of C. indica, and to evaluate the removal rates of nutrients in water body by C. indica inoculated with endophytic bacteria in the pilot scale floating bed treatment system. The results showed that C01, C16 and C20 promoted the germination of seed and growth of plants. The germination rate of C16 treatment was 83.3%, significantly higher than that of the control, which was 31.1%. During the water purification experimental stage, the removal rates of total phosphorus and total nitrogen in the C16 treatment were 88.4% and 96.9%, respectively, which were 26.4% and 15.6% higher than the control. At the end of the experiment, the biomass and root length of the C01 and C16 treatment were significantly higher than the control, the soluble sugar content and soluble protein content in the C16 treatment were 317.3% and 97.8% higher than those in the control respectively. C01, C16 and C20 were proved to be Pseudomoanas fluorescens, Enterobacter sp., Erwinia sp. based on 16S rRNA sequence analysis.