补骨脂对大鼠正畸后稳定性的影响

Effect of psoralen on the stability after orthodontic tooth movement in rats

目的:探讨补骨脂素对大鼠正畸牙移动后牙周组织改建的影响.方法:选择36只8周龄雄性Wistar大鼠,将其随机分为实验组和对照组2组.在上颌左侧切牙和上颌左侧第一磨牙之间安装拉第一磨牙向近中的加力装置.加力21 d后去除装置,对2组进行药物灌胃处理.实验组大鼠灌服8 mg/(kg·d)补骨脂素溶液,对照组大鼠每天灌服等量生理盐水,连续灌服4周.在去除装置当天和第7、14、21、28天制取大鼠上颌石膏模型,3D扫描获得电子数据,测量并计算复发距离.灌药4周后,心脏灌注处死全部大鼠,分离上颌骨,分离大鼠磨牙及其周围的牙周组织,对切片进行H-E染色和BMP2、BMP4免疫组织化学染色.采用SPSS 21.0软件包对数据进行统计学分析.结果:拆除矫治器后,2组均有复发,实验组在第7、14、21、28天的复发率显著小于对照组(P<0.05).2组复发速度均在第1周最快,第2、3、4周逐渐变慢,实验组在第1周复发速度显著小于对照组(P<0.05),2组牙周膜和牙槽骨中均有BMP2、BMP4表达,实验组表达强度高于对照组(P<0.05).结论:补骨脂素能够促进牙周组织中BMP2、BMP4的表达,加速牙周组织改建,有效抑制复发.

PURPOSE: This study was designed to investigate the effect of psoralen on periodontal tissue reconstruction after orthodontic tooth movement (OTM) in rats. METHODS: Thirty -six male 6 -week -old Wistar rats were randomly divided into 2 groups: the experimental group and the control group. The experimental group and the control group were all installed between the central incisor and the left maxillary first molars to pull the first molars away from the force device;after 21 days, the force was removed and the rats in 2 groups were given drug gavage. Rats in the experimental group were given a gavage of psoralen 8 mg/kg per day, while rats in the control group were given the same amount of 0.9% sodium chloride everyday. Maxillary casts were made every week during the experimental and were scanned by 3D Scanner to measure relapse distance, and histologic examination was conducted. After 28 days, the rats were sacrificed and rats' upper jaw was separated. The remaining sections were immunohistochemically stained with BMP2 and BMP4. SPSS 19.0 software package was used for statistical analysis. RESULTS: Both groups had relapse after the force device was removed. Significant decrease of relapse percentage was observed in the experimental group compared with the control group at day 7 ,day 14, day 21 and day 28(P<0.05 ). The speed of relapse of both groups were fastest in the first week and slowed down in the second, third and fourth week gradually. The speed of relapse in the experimental group in the first week was significantly less than in the control group (P<0.05).The expression of BMP2 and BMP4 within periodontal membrane and alveolar bone was significantly higher in the experimental group than in the control group (P <0.05). CONCLUSIONS: Psoralen can accelerate the reconstruction of periodontal tissues of orthodontic tooth and reduce relapse.