原发性干燥综合征线粒体功能相关基因表达的转录组学分析

Transcriptome analysis of mitochondrial function related gene expression in primary Sjogren′s syndrome

目的通过分析线粒体功能相关基因在原发性干燥综合征(pSS)患者和正常组的差异表达情况来研究线粒体功能的异常与pSS之间的关系。方法对GEO数据库中来源于pSS患者和正常组细胞毒性CD8+T细胞样本的基因芯片表达谱数据采用t检验和FC(foldchange)差异分析方法筛选出差异表达基因,并对差异表达基因进行功能富集、功能注释以及蛋白质互作网络分析。结果基于pSS组和正常组共筛选出347个差异表达的基因,其中61个为线粒体相关基因,1个为线粒体基因。进一步分析发现这62个线粒体功能相关基因主要涉及细胞能量供应、线粒体基因转录和翻译、核苷酸代谢以及细胞色素C的释放等过程。结论pSS患者中部分线粒体功能相关基因的失调影响了线粒体功能,提示线粒体功能的异常与pSS的发生发展相关。

Objective To investigate the relationship between mitochondrial function abnormality and primary Sjogren′s syndrome (pSS) by analyzing the expression difference of mitochondrial function related genes between pSS patients and normal control. Methods The gene expression profiles data of cytotoxic CD8 +T cells samples from pSS patients and normal group in GEO database were analyzed by adopting the t test and FC (Fold Change) differential analysis.The functional annotation,functional enrichment and protein interaction network analysis of differently expressed genes (DEGs) were performed. Results A total of 347 DEGs were screened out based on the pSS and normal groups.Among them,61 DEGs were mitochondria related genes and one was the mitochondrial gene.The further analysis revealed that these 62 mitochondrial function related genes were mainly involved in the processes of cell energy supply,mitochondrial gene transcription and translation,nucleotide metabolism and release of cytochrome C. Conclusion The dysregulation of partial mitochondrial function related genes in pSS patients affects the mitochondria function,suggesting that the abnormality of mitochondrial function may be associated with the occurrence and development of pSS.