葛根素对2型糖尿病大鼠的治疗作用

Effects of puerarin on rats with type 2 diabetes mellitus

目的:观察葛根素对2型糖尿病(T2DM)大鼠的治疗作用。方法:采用高糖高脂饲料喂养加一次性腹腔注射60 mg/kg链脲佐菌素的方法建立T2DM大鼠模型,随机分为正常组,模型组,二甲双胍(40 mg/kg)组,葛根素低、中、高剂量(40,80,160 mg/kg)组,每组10只大鼠;造模成功后,灌胃给药4周,每周测量大鼠体重和空腹血糖(FBG),末次给药24 h后取血,收集血清,检测各组大鼠的血糖、血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白-胆固醇(LDL-C)水平、高密度脂蛋白-胆固醇(HDL-C),血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活性,血清尿素氮(BUN)、肌酐(SCr)、尿酸(UA)水平。结果:干预4周后,与正常组比较,模型组大鼠体重显著降低(P<0.01),FBG,TC,TG,LDL-C,ALT,AST,BUN,SCr,UA均显著升高(P<0.01),而HDL-C显著降低(P<0.01);与模型组比较,二甲双胍组和葛根素各剂量组大鼠体重均显著增加(P<0.01),FBG,TC,TG,LDL-C,ALT,AST,BUN,SCr,UA均显著降低(P<0.01),而HDL-C显著升高(P<0.01)。结论:葛根素能够减少T2DM大鼠体重降低幅度,降低血脂、血糖水平,可用于T2DM的治疗。

Objective: To investigate the therapeutic effects of puerarin on rats with type 2 diabetes mellitus( T2 DM). Methods: T2 DM models were established by high fat and high glucose feeding combined with a one-time intraperitoneal injection of streptozotocin( STZ,60 mg/kg). Then the rats were randomly divided into normal group,model group,metformin group( MET,40 mg/kg),puerarin low-dose group,medium-dose group and high-dose group( 40,80,160 mg/kg),n = 10. After the model was successfully established,rats were treated with corresponding drug intervention by intragastrical administration for 4 weeks. The body weight and fasting blood glucose( FBG) were measured per week,and blood samples were collected 24 h after the last administration,and serum levels of blood glucose,serum triglyceride( TG),total cholesterol( TC),low-density lipoprotein-cholesterol( LDL-C),high-density lipoprotein-cholestrol( HDL-C),serum enzyme activities of aspartate aminotransferase( AST),alanine aminotransferase( ALT),blood urea nitrogen( BUN),serum creatinine( SCr),and blood uric acid( UA) were measured. Results: As compared with normal group,the body weight was decreased after 4 weeks-intervention in the model group,and the levels of FBG,TC,TG,LDL-C,ALT,AST,BUN,SCr and UA were all increased,while HDL-C level was decreased( P<0. 05). As compared with model group,the body weight was increased after 4 weeks-intervention in metformin group and puerarin groups,and the levels of FBG,TC,TG,LDL-C,ALT,AST,BUN,SCr and UA were decreased( P<0. 01);meanwhile,HDL-C level was increased significantly( P<0. 05). Conclusion: Puerarin can reduce the weight loss of T2 DM rats,decrease the blood lipid and blood glucose levels of T2 DM rats,which can be used to control T2 DM.