石菖蒲及其活性成分-5-羟甲基糠醛对疲劳运动大鼠学习记忆的影响及其机制

Effects of Acorus tatarinowii Schott and its active component-5-hydroxymethylfurfural on ERK/CREB signal in hippocampus of exercise-induced fatigue rats

目的:研究石菖蒲及5-羟甲基糠醛(HMF)对疲劳运动大鼠学习记忆和海马ERK/CREB信号的影响。方法:将SD大鼠随机分为:正常组(A)、运动组(B)、运动+HMF低、中、高剂量组(C、D、E)、运动+石菖蒲低、中、高剂量组(F、G、H),每组10只。并在疲劳运动开始前2 h分别以0.10、1.00和3.00 mg.kg^-1·wt^-1HMF,灌胃C、D、E组,以0.12、1.20和4.80 g.kg^-1·wt^-1石菖蒲提取物,灌胃F、G、H组。实验结束后采用水迷宫实验进行学习记忆检测,用免疫印迹法测定海马p-ERK1/2和p-CREB表达。结果:E和H组大鼠逃避潜伏期低于B、C、D、F和G组;穿越平台次数、p-ERK1/2、p-CREB蛋白表达高于B、C、D、F和G组(P均<0.01);除E组p-ERK2蛋白表达低于A、H组(P<0.05)外,上述各指标A、E、H组比较,差异均无显著性(P均>0.05)。结论:石菖蒲及HMF能明显改善运动疲劳大鼠学习记忆,其机制与上调海马ERK/CREB信号有关。

Objective: To investigate the effects of Acorus tatarinowii Schott and its active component 5-hydroxymethyl furfural( HMF) on learning and memory and ERK/CREB signal in hippocampus of rats with exercise-induced fatigue. Methods: SD rats were randomly divided into normal group( A),exercise group( B),exercise + HMF low,middle and high dose treatment group( C,D,E),exercise + acorus tatarinowii Schott low,middle and high dose treatment group( F,G,H),with ten rats in each group. The rats in group C,D and E were treated with HMF at the doses of 0. 10,1. 00 and 3. 00 mg. kg^-1 by ig. The rats in group F,G and H were treated with the extracts of Acorus tatarinowii Schott at the doses of 0. 12,1. 20 and 4. 80 g. kg^-1 by ig. Learning and memory of rats were tested by the method of water maze experiment,and the expression levels of p-ERK1/2 and p-CREB protein in hippocampus of rats were tested by the method of Western blot in the end of the experiment. Results: The escape latencies of E and H groups were lower than those of groups B,C,D,F and G;and the numbers of plateau crossing were more than those of groups B,C,D,F and G and the expression levels of p-ERK1/2,p-CREB protein were higher than those of groups B,C,D,F and G,respectively( P < 0. 01).There was no significant difference in the above indexes among groups A,E and H( P>0. 05) except that the expression levels of pERK2 protein in group E were lower than those in group A and H( P<0. 05). Conclusion: Acorus tatarinowii and its active component-HMF can improve the learning and memory of rats with exercise-induced fatigue,and the mechanism is related to the up-regulation of ERK/CREB signal in hippocampus of rats with exercise-induced fatigue.