醒鼻凝胶滴鼻剂对变应性鼻炎豚鼠Periostin、IRE1α及嗅区鼻黏膜形态学的影响

Effect of Traditional Chinese Medicine Xingbi Gelation Nasal Drops on Periostin,IRE1α and Morphological Effects of Nasal Mucosa in Olfactory Region in Allergic Rhinitis Guinea Pigs

观察醒鼻凝胶滴鼻剂对AR(变应性鼻炎,allergic rhinitis)豚鼠鼻黏膜及血清Periostin(骨膜素,Pn)、IRE1α(内质网感应蛋白肌醇需求酶,1α)的影响,扫描电镜观察其对嗅区鼻黏膜形态学的影响,联合血清Ig E(免疫球蛋白E,immunoglobulin E)及变应性鼻炎豚鼠行为学评分,进行醒鼻凝胶滴鼻剂的机制研究。健康普通级豚鼠70只,OVA(卵清蛋白,Ovalbumin)致敏建立变应性鼻炎豚鼠模型,随机分为完全空白对照组;生理盐水模型组;生理盐水给药模型组,醒鼻凝胶滴鼻剂低、中、高剂量组,雷诺考特组,10只/组。观察各组行为学变化,酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测血清Periostin、Ig E、IRE1α;逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测鼻黏膜Periostin、IRE1αm RNA,扫描电镜观察嗅区鼻黏膜形态。结果显示:AR豚鼠模型构建成功;OVA模型组鼻黏膜Periostin m RNA表达上升(P <0. 05),鼻黏膜IRE1αm RNA及血清Periostin、IRE1α、Ig E表达也均上升(P <0. 01);高剂量醒鼻凝胶滴鼻剂给药后鼻黏膜及血清中Periostin、IRE1α、Ig E表达均显著下调(P <0. 01);OVA模型组嗅区黏膜细胞管腔萎缩,脱落、变稀,排列紊乱,组织表面粘连,见较多分泌物附着;醒鼻凝胶滴鼻剂干预后嗅区鼻黏膜细胞管腔再生,排列较整齐,分泌物减少。醒鼻凝胶滴鼻剂可下调鼻黏膜及血清Periostin、IRE1α的表达,影响血清Ig E,并能减轻炎症分泌物,促进变应性鼻炎豚鼠嗅区鼻黏膜细胞管腔再生,发挥治疗变应性鼻炎的作用,且存在一定浓度依赖,高浓度药效更佳。

To observe the effect of traditional chinese medicine xingbi gelation nasal drops on the expression of periostin,IRE1α in nasal mucosa and serum of guinea pigs with allergic rhinitis (AR),scanning electron microscope observation on morphology of nasal mucosa in olfactory region,combined with serum IgE and behavioral scores of allergic rhinitis guinea pigs,the mechanism of rhingel nasal drops was studied.The allergic rhinitis guinea pig model was established by ovalbumin(OVA)sensitization,70 healthy guinea pigs were random divided into blank control group (group A),saline group (group B),normal saline control group (group C),traditional chinese medicine xingbi gelation nasal drops low dose group (D),middle dose group (E),high dose group (F),Ray Naukauot (G) group,10/group. The OVA model was established in C,D,E,F and G groupsl,while group B was saline model.Behavioral changes were observed in each group,the expression of IRE1α in serum was detected by ELISA,the expression of IRE1 α in nasal mucosa was detected by RT-PCR,observation of nasal mucosa morphology in olfactory region by scanning electron microscopy. AR guinea pig model is successfully constructed.In the ova model group,the levels of periostin mRNA in the nasal mucosa increased ( P <0.05),IRE1α mRNA in the nasal mucosa and periostin,IRE1α and IgE in serum increased ( P <0.01). The expression of periostin,IRE1α and IgE in serum and nasal mucosa tissues is significantly decreased after high dose of nasal gel nasal drops ( P <0.01). In the OVA model group,the mucosal cells of the olfactory area are atrophic,shedding,thinning,arranged in disorder,the surface of the tissue adhered,and more secretions are attached. After the intervention of traditional chinese medicine xingbi gelation nasal drops,the nasal cavity cells in the olfactory area are regenerated,arranged orderly,and secretions decreased.Traditional Chinese medicine Xingbi gelation nasal drops can reduce the expression of periostin,IRE1α in nasal mucosa and serum,influence IgE in serum,reduce inflammatory secretions,promote the regeneration of nasal mucosa cells in allergic rhinitis guinea pigs,and play a role in the treatment of allergic rhinitis.And there is a concentration dependent,medium and high concentration in better efficacy.