变波长高效液相色谱法同时测定酱腌菜中的安赛蜜和脱氢乙酸

Simultaneous determination of acesulfame potassium and dehydroacetic acid in pickled vegetables by high performance liquid chromatography with programmed wavelength UV detection

建立了一种同时测定酱腌菜中安赛蜜和脱氢乙酸的变波长高效液相色谱检测方法。样品中安赛蜜和脱氢乙酸用水提取,硫酸锌和氢氧化钠沉淀蛋白,经C18色谱柱(250×4.6mm,5μm)分离,程序化变波长检测,外标法定量。在设定的条件下,安赛蜜、脱氢乙酸分别在0.4~80mg/L、1~200mg/L浓度范围内呈现良好的线性关系(R2>0.9995),定量限分别为2.0mg/kg、5.0mg/kg。在低、中、高3个添加水平下,安赛蜜、脱氢乙酸的平均回收率分别在96.0%~102.3%之间和97.8%~101.7%之间,相对标准偏差分别在0.5%~2.2%之间和0.6%~1.8%之间。实验结果表明该方法简单快捷、结果准确、重现性良好,适用于酱腌菜中安赛蜜和脱氢乙酸含量的测定。

In this paper,a method of simultaneous determination of acesulfame potassium and dehydroacetic acid in pickled vegetables by high performance liquid chromatography (HPLC) with programmed wavelength UV detection was developed.Acesulfame potassium and dehydroacetic acid in samples were extracted in water (the protein was precipitated by zinc sulfate and sodium hydrate subsequently),separated by Agilent ZORBAX XDB-C18 (250×4.6mm,5μm),determined by variable wavelength detector,and quantified by external standard curve.Under the experimental conditions,acesulfame potassium and dehydroacetic acid had a good resolution and well linearity( R2 > 0.9995) in detection range respectively.The limits of quantification for acesulfame potassium and dehydroacetic acid were 2.0 mg/kg and 5.0 mg/kg respectively.The average recoveries from spiked samples ranged from 96.0% to102.3% and from 97.8% to 101.7% with relative standard deviation (RSD) between 0.5% and 2.2%,and 0.6% and 1.8% respectively.With the advantages of convenience,accuracy and good repeatability,the method was suitable for the detection of acesulfame potassium and dehydroacetic acid in pickled vegetables.