马氏珠母贝精子低温条件下的活力保鲜期

The Quality Guarantee Period of Spermatozoa from Pinctada fucatamartensii Sperm under Refrigerator Temperature Condition

以Ca-freeHBSS作为精子基础稀释剂及短期冷藏保存液,在4℃低温条件下,对马氏珠母贝的新鲜精子进行短期冷藏,连续取样对精子活力及运动能力进行检测,研究马氏珠母贝新鲜精子的活力“保鲜期”.以2mmol/L人工氨海水作为马氏珠母贝精子运动激活剂,激活30s后用精子质量分析仪对其运动率及游泳运动参数进行检测.结果表明:4℃条件下,马氏珠母贝精子低温冷藏活力有效期为24h,冷藏期间精子的总运动率为70%、直线运动速率为126μm/s、曲线运动速率为106μm/s、平均位移速率为125μm/s、头部位移距离为1.76μm、鞭毛摆动频率为9Hz,具有较好的活力.

With Ca-free Hanks’ balanced salt solution (Ca-free HBSS) used as basic diluent extender and storage medium,fresh sperm from Pinctada fucatamartensiiwere stored at4 ℃,and the sperm motility and swimming ability were analyzed continuously to investigate the guarantee period of sperm activity.With seawater containing2 mmol/L ammonia used as activated solution,sperm motility and swimming ability were analyzed by sperm class analyzer30 s later after the activation of the sperm.The results indicated that at4 ℃,during the guarantee period of sperm activity which is less than24 h,the sperm have high activity-with 70% total motile sperm,126 μm/s straight-line velocity,106 μm/s curvilinear velocity,125 μm/s average path velocity,1.76 μm head displacement and 9 Hz beat-cross frequency.