临床生化检验中关于血液标本放置时间对检验结果的影响

The Effect of Blood Specimen Placement Time on the Test Results in Clinical Biochemical Tests

目的:探究血液标本放置时间在临床生化检验中对检验结果产生的影响。方法:本次试验对象均为2018年2月-2019年3月在笔者所在医院进行生化检验的95例患者,采集血液标本1、4、8 h后进行生化检验,记录并分析γ谷氨酰转肽酶、钾离子、甘油三酯、碱性磷酸酶、谷丙转氨酶、血糖及总胆红素水平。结果:标本放置1 h检测的γ谷氨酰转肽酶、钾离子、甘油三酯、碱性磷酸酶及总胆红素水平与放置4 h比较,差异均无统计学意义(P>0.05);标本放置1 h检测的谷丙转氨酶水平低于放置4 h,血糖水平高于放置4 h,差异均有统计学意义(P<0.05)。标本放置4 h检测的γ谷氨酰转肽酶、甘油三酯、碱性磷酸酶、总胆红素、血糖水平均高于放置8 h,钾离子、谷丙转氨酶水平均低于放置8 h,差异均有统计学意义(P<0.05)。结论:在临床生化检验中,血液标本放置时间对检验结果产生的影响较大,因此需合理安排检测顺序,缩短检验时间,以减少和预防检测结果的不准确性。

Objective: To investigate the effect of blood specimen placement time on the test results in clinical biochemical tests. Method: The subjects were selected from 95 patients who underwent biochemical tests in our hospital from February 2018 to March 2019. Biochemical tests were performed at 1, 4, and 8 h after specimen collection, and gamma-glutamyl transpeptidase, potassium ion, triglyceride, alkaline phosphatase, alanine aminotransferase, blood glucose and total bilirubin levels were recorded and analyzed. Result: The levels of gamma-glutamyl transpeptidase,potassium ion, triglyceride,alkaline phosphatase and total bilirubin were compared between 1 hour and 4 hours after specimen collection,and the differences were not statistically significant (P>0.05). But the level of alanine aminotransferase at 1 h after specimen placement was lower than that of 4 h after specimen placement,and blood glucose levels was higher than that of 4 h after specimen placement, and the differences were statistically significant (P<0.05). The levels of gamma-glutamyl transpeptidase, triglyceride, alkaline phosphatase, total bilirubin and blood glucose at 4 h after specimen placement were higher than those of 8 h after specimen placement,and the levels of potassium ion and alanine aminotransferase were lower than those of 8 h after specimen placement, and the differences were statistically significant (P<0.05). Conclusion: The blood specimen placement time has a great influence on the test results in clinical biochemical tests. Therefore, it is necessary to arrange the test sequence reasonably and shorten the test time to reduce and prevent the inaccuracy of the test results.