外周血T细胞亚群和血浆血管内皮生长因子在慢性肾小球肾炎患者中的表达及临床意义

The study of the expression and clinical significance of peripheral blood T cell subsets and plasma vascular endothelial growth factor in patients with chronic glomerulonephritis

目的分析外周血T细胞亚群和血浆血管内皮生长因子(VEGF)在慢性肾小球肾炎(CGN)患者中的表达及临床意义。方法选取2016年6月~2018年5月我院收治的62例CGN患者与44例健康志愿者作为研究对象,检测所有人员外周血T细胞亚群与VEGF表达水平,采用酶联免疫法(ELISA)检测VEGF,单克隆抗体法检测外周血T细胞亚群。分析影响外周血T细胞亚群与VEGF表达水平的因素与病情程度的相关性。结果两组在T-淋巴细胞亚群百分率与VEGF表达水平比较中,CGN组CD3^+、CD4^+、CD4^+/CD8^+均明低于对照组,VEGF表达水平明显高于对照组(P<0.05);在62例CGN患者中,其中,轻型40例,占比64.52%,重型24例,占比38.71%(P<0.05)。在T细胞亚群表达水平比较中,重型CGN患者CD3^+、CD4^+、CD4^+/CD8^+各值较轻型CGN明显降低,VEGF水平明显升高,数据间差异具有统计学意义(P<0.05);Spearman相关性分析结果提示,CD3^+、CD4^+、CD4^+/CD8^+表达水平与CGN疾病分型呈现负相关(P<0.05),VEGF表达水平与CGN疾病分型呈现正相关(P<0.05)。结论 CGN患者外周血T细胞亚群表达水平明显低于正常,血浆VEGF水平明显高于正常,且T细胞亚群各因子表达水平与CGN病情严重程度呈现负相关,VEGF表达水平与患者病情呈现正相关。因此,在该疾病治疗中,免疫力的提高与VEGF的控制具有重要意义。

Objective To analyze the expression and clinical significance of peripheral blood T cell subsets and serum vascular endothelial growth factor(VEGF) in the patients with chronic glomerulonephritis(CGN). Methods 62 CGN patients and 44 healthy volunteers admitted to our hospital from June 2016 to May 2018 were selected as the study subjects. The expression levels of T cell subsets and VEGF in peripheral blood of all the staff were detected. VEGF was detected by enzyme-linked immunoassay(ELISA). T cell subsets in peripheral blood were detected by monoclonal antibody. The correlation between factors influencing the expression level of peripheral blood T cell subsets and VEGF and the severity of the disease were analyzed. Results CD3^+, CD4^+ and CD4^+/CD8^+ in the CGN group were significantly lower than those in the control group(P<0.05). The expression levels of VEGF in the CGN group were significantly higher than those in the control group(P<0.05). Among the 62 patients with CGN, 40 were mild(64.52%), and 24 were severe(38.71%). CD3^+, CD4^+, CD4^+/CD8^+ values of patients with severe CGN were significantly lower than those of patients with mild CGN, and VEGF levels were significantly increased(P<0.05). Spearman correlation analysis showed that CD3^+, CD4^+, CD4^+/CD8^+ expression levels were negatively correlated with CGN disease typing(P<0.05), and VEGF expression level was positively correlated with CGN disease typing(P<0.05). Conclusion Peripheral blood T cell subsets and plasma VEGF were highly expressed in patients with CGN, and the expression levels of various factors in the T cell subgroup were negatively correlated with the severity of CGN, while VEGF expression levels were positively correlated with patients with CGN. Therefore, the improvement of immunity and the control of VEGF are of great significance in the treatment of this disease.