支气管哮喘患者的血清分泌型卷曲相关蛋白5与其气道炎症的相关性分析

Correlation between serum secreted frizzled-related protein 5 and airway inflammation in patients with bronchial asthma

目的探讨支气管哮喘(哮喘)患者的血清分泌型卷曲相关蛋白5(SFRP5)与其气道炎症的相关性.方法选取哮喘患者96例,分为急性组和缓解组,每组48例,选择同时期进行健康体检者48例作为正常对照组,用酶联免疫吸附试验(ELISA)法检测血清中SFRP5、转化生子因子-β1(TGF-β1)、白细胞介素-13(IL-13)、肿瘤坏死因子-α(TNF-α)水平,同时进行肺功能检测,进行相关分析.结果缓解组和急性组SFRP5、第1秒用力呼气容积占预计值百分比(FEV1%pred)和FEV1/FVC均低于对照组,TGF-β1、IL-13和TNF-α水平均高于对照组,差异有统计学意义(F=38.24、5.81、3.54、41.53、8.37、63.84,P值均<0.05);急性组SFRP5、FEV1%pred和FEV1/FVC均低于缓解组,TGF-β1、IL-13和TNF-α水平均高于缓解组,差异有统计学意义(t=9.842、25.199、6.265、15.365、16.236、19.040,P值均<0.05);中重度急性组SFRP5、FEV1%pred和FEV1/FVC均低于轻度急性组,T G F-β1、IL-13和T N F-α水平均高于轻度急性组,差异有统计学意义(t=3.96、30.62、24.96、5.08、7.47、9.58,P值均<0.05);哮喘患者血清中SFRP5水平与TGF-β1、IL-13和T N F-α呈负相关,差异有统计学意义(r=-0.459、-0.518、-0.642,P值均<0.05);与FEV1%pred、FEV1/FVC和病情呈正相关,差异有统计学意义(r=0.452、0.4788、0.472,P值均<0.05).结论 SFRP5与气道炎症呈负相关,与肺功能和病情呈正相关,SFRP5作为一种抗炎因子,对哮喘患者病情严重程度及预后判断、治疗具有重要意义.

Objective To investigate the correlation between serum secreted frizzled-related protein 5 ( SFRP5) and airway inflammation in patients with bronchial asthma ( asthma).Methods Ninety-six patients with asthma were selected and divided into acute group and remission group , with 48 cases in each group .Another 48 healthy individuals were set as control group .Serum SFRP5 ,transforming growth factor-β1 ( TGF-β1 ),interleukin-13 ( IL-13),and tumor necrosis factor-α( TNF-α) levels were detected by enzyme-linked immunosorbent assay ( ELISA ). Moreover ,the pulmonary function was also detected .Then the correlation of the serum levels with the pulmonary function was analyzed .Results The SFRP5 ,forced expiratory volume in one second as a percentage of expected value ( FEV1% pred) and FEV1/FVC in remission group and acute group were lower than those of in control group ,and the levels of TGF-β1 ,IL-13 and TNF-α were higher than those of in control group ,and the difference was statistically significant ( F =38 .24 ,5 .81 ,3 .54 ,41 .53 ,8 .37 ,63 .84 ,all P < 0 .05).The SFRP5 ,FEV1% pred and FEV1/FVC in acute group were lower than those of in remission group ,and the levels of TGF-β1 ,IL-13 and TNF-αwere higher than those of in remission group ,and the difference was statistically significant ( t =9 .842 , 25 .199 ,6 .265 ,15 .365 ,16 .236 ,19 .040 ,all P <0 .05).The SFRP5 ,FEV1% pred and FEV1/FVC in moderate and severe acute group were lower than those of in mild acute group ,and the levels of TGF-β1 ,IL-13 and TNF-α were higher than those of in mild acute group ,and the difference was statistically significant ( t = 3 .96 ,30 .62 ,24 .96 ,5 .08 ,7 .47 ,9 .58 ,all P < 0 .05 ).Serum SFRP5 level was negatively correlated with TGF-β1 ,IL-13 and TNF-α in patients with asthma ,and the difference was statistically significant ( r =-0 .459 ,-0 .518 ,-0 .642 ,all P < 0 .05 ).Serum SFRP5 level was positively correlated with the FEV 1% pred ,FEV1/FVC and condition ,and the difference was statistically significant ( r = 0 .452 ,0 .4788 ,0 .472 ,all P < 0 .05).Conclusions SFRP5 level is negatively correlated with airway inflammation and positively correlated with lung function and condition .As an anti-inflammatory factor ,SFRP5 has important predictive significance for the severity ,the prognosis and treatment of the asthma patients .