我国黄热病疫苗株(天坛株)与WHO疫苗株17D-213的安全性研究

Safety of yellow fever vaccine strain (Tiantan strain) in China and WHO vaccine strain 17D-213

目的研究我国黄热病疫苗株天坛株与WHO疫苗株17D-213在小鼠体内的脑内致病力。方法将同等病毒量的天坛株与17D-213株脑内注射不同年龄、不同品系的小鼠,观察并记录小鼠生存情况,分析两毒株的LD50/ml以及半数生存期。结果同等病毒量的天坛株与17D-213株对1日龄乳鼠、7~9 g小鼠、12~14 g小鼠的半数致死量(LD50)/ml均无差异,对7~9 g小鼠与12~14 g小鼠的生存趋势差异也无统计学意义。两病毒株对1日龄乳鼠的生存趋势存在一定的统计学差异,但是天坛株的半数生存期为11 d,WHO疫苗株17D-213为6 d。同等病毒量的两毒株,对BALB/c、KM、ICR、C57四个品系小鼠的LD50/ml也均无差异,仅对NIH小鼠表现出有一定的差异。结论我国黄热病疫苗株天坛株与WHO疫苗株17D-213对不同年龄、不同品系小鼠的脑内致病力无明显差异,安全性良好。

Objective To study the intracerebral pathogenicity of the yellow fever vaccine strains of Tiantan strain used in China and WHO vaccine strain 17D-213 in mice. Methods Mice of different ages and strains were intracerebrally injected with same amount of Tiantan strain and 17D-213 strain. The death and survival of mice were observed and recorded. The LD50/ml and half survival time of the two vaccine strains were compared and analyzed. Results There was no difference in LD50/ml between the Tiantan strain and 17D-213 strain when used through intracerebral injection in one-day-old suckling mice, 7-9 g mice or 12-14 g mice. Moreover, no significant difference in survival trend was found in 7-9 g mice or 12-14 g mice injected with the two vaccine strains. However, the two strains had statistically different influences on the survival trend of one-day-old suckling mice. The half survival time of the Tiantan strain was 11 d, while that of the WHO vaccine strain 17D-213 was 6 d. Excepting in NIH mice, no significant differences in LD50/ml were detected between the same amount of two strains in BALB/c, KM, ICR or C57 mice. Conclusions The yellow fever Tiantan vaccine strain and WHO vaccine strain 17D-213 have no significant difference in the intracerebral pathogenicity in mice of different ages and strains with good safety.