未成熟卵体外培养与印记基因甲基化研究进展

Advances in imprinted DNA methylation of in vitro culture of immature oocytes

卵母细胞体外成熟(IVM)是一项试管婴儿领域的前沿技术,适用于缺乏成熟卵母细胞的患者,已被应用到临床辅助生殖技术中.它可以减少促性腺激素对患者的刺激,消除多囊卵巢综合征(PCOS)患者出现卵巢过度刺激综合征(OHSS)的风险,具有减少治疗负担和成本的优势.但值得关注的是,卵母细胞的培养可能会干扰发育过程中的表观重新编程,导致子代出生缺陷.本文将从IVM对印记基因表达、早期胚胎发育能力、印记基因甲基化的影响以及胚胎培养对甲基化的影响等方面做一综述.

In vitro maturation (IVM) of oocytes, a frontier technique in in vitro fertilization (IVF) field, specifically targeting at some infertile patients with egg maturation disorders, have been introduced into the clinical assisted reproductive technology. It is a mild approach to replace conventional ovarian stimulation for polycystic ovary syndrome (PCOS) patients and has advantages of reducing the burden and cost of treatment. However, it is noteworthy that oocyte culture may interfere with epigenetic reprogramming of the development process, leading to birth defects in the offspring. This review will talk about impression of IVM on expression of imprinted genes, early embryonic development and imprinted gene methylation and impression of embryo culture on the methylation.