实时荧光定量聚合酶链式反应技术在B族链球菌检测中的应用研究进展

Application of real-time fluorescence quantitative polymerase chain reaction in detection of group B streptococcus

B族链球菌(GBS)是围产期孕妇感染导致新生儿感染致病的主要致病菌之一,可引起新生儿肺炎、败血症、脑膜炎等早期侵入性感染,严重的甚至会导致死亡.本文综述了金标准培养法、快速培养法与实时荧光定量聚合酶链式反应(PCR)法在孕产妇检测GBS阳性率、敏感度和特异度方面的差异,对不同孕周的孕妇采用何种检测方法提出了建议,以期能够在全国范围内普及GBS的筛查和预防,达到降低母婴并发症的目的.

Group B Streptococcus (GBS) is one of the main pathogens causing neonatal infections in perinatal pregnant women, which can cause early invasive infections such as neonatal pneumonia, sepsis, meningitis, and even serious death. This article reviews the differences between the gold standard culture method, the rapid culture method and the real-time fluorescent quantitative polymerase chain reaction (PCR) method in the positive rate, sensitivity and specificity of maternal detection of GBS, and puts forward some suggestions for the detection methods used by pregnant women in different gestational weeks. This review suggested that screening and prevention of GBS nationwide will reduce maternal and child complications.