羊水细胞传代再培养在产前诊断性状异常羊水孕妇中的应用

Application of amniotic fluid cell inheritance and re-culture in cases of amniotic fluid with abnormal traits

目的探讨羊水细胞传代再培养在性状异常羊水孕妇诊断中的应用价值.方法选取安徽医科大学第一附属医院2014年1月至2018年12月行羊膜腔穿刺术中性状异常羊水孕妇15例,常规接种并在培养10 d后分瓶传代再培养,收获后计数细胞数量并行核型分析.结果 15例羊水标本中7例羊水色略深, 3例羊水呈洗肉水样,5例羊水呈浅褐色或褐色. 15例标本原代细胞瓶镜下细胞数(2.40 ±5.87)×10^5/mL,传代细胞瓶镜下细胞数(2.76 ±0.64)×10^6/mL,差异有统计学意义(t=-10.825,P<0.05).传代细胞瓶15例标本核型分析均获得满意结果.结论羊水细胞传代再培养技术能够增加性状异常羊水培养后细胞的数量,提高染色体核型分析成功率.

Objective To evaluate amniotic fluid cell inheritance and re-culture in cases of amniotic fluid with abnormal traits. Methods From January 2014 to December 2018, 15 cases of amniotic fluid with abnormal traits in the First Affiliated Hospital of Anhui Medical University were selected in amniocentesis.Amniotic fluid cells were routinely seeded and cultured for 10 days, then subcultured into other culture bottles.The number of cells and karyotyping after harvesting were counted. Results Seven of 15 cases of amniotic fluid color were slightly darker, 3 cases were pink as water washed meat, and 5 cases were light brown or brown.The average number of cells in original bottles was (2.40±5.87)×105/mL, the average number of cells in inheritance bottles was (2.76±0.64)×106/mL.All 15 samples in the cell inheritance bottles got satisfactory results in cell karyotype analysis. Conclusion Amniotic fluid cell inheritance and re-culture can increase the number of cells in amniotic fluid cell culture and improve the success rate of karyotyping.