摘要
从江香猪是全国稀有的微型猪种,其细胞色素氧化酶P450 基因纯合度较高,遗传稳定,具备开发药物代谢动物模型的良好基础。本研究旨在应用实时荧光定量方法研究从江香猪细胞色素氧化酶P4503A29 mRNA 的组织分布及地塞米松对其表达的影响,并比较与人细胞色素氧化酶P450 3A4 的相似性。结果表明,从江香猪细胞色素氧化酶P450 3A29 mRNA 的组织分布特征及地塞米松对其诱导作用总体上与人体相似,从江香猪可用于人细胞色素氧化酶P450 3A4 的诱导研究。
Congjiang Xiang-pig is a rare species of miniature pig in China. It has a good basis for animal model of drug metabolism because of its highly homozygous and stable heredity of P450 genes. The aim of this study is to analyze the tissue distribution and the effect of dexamethasone on the expression of Congjiang Xiang-pigs CYP3A29 mRNA by real-time RT-PCR, and compare the similarity with that of human CYP3A4. The results showed that the characters of tissue distribution and the induction effect of dexamethasone on the expression of CYP3A29 mRNA in Congjiang Xiang-pigs were similar to those of human beings, and Congjiang Xiang-pig could be used in the induction study of human CYP3A4.
作者
杨家大
吴声榕
Yang Jiada;Wu Shengrong(School of Life and Health Science,Kaili University,Kaili,556011;Library of Kaili University,Kaili,556011)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2019年第9期3994-4000,共7页
Genomics and Applied Biology
基金
贵州省科技厅、黔东南州科技局、凯里学院科技合作协议项目(黔科合LH字[2017]7175号)
国家自然科学基金(81460572)共同资助
