心脏瓣膜病中线粒体凋亡调控因子与microRNA的相互作用

Interaction of Mitochondrial Apoptosis Regulators with microRNAs in Valvular Heart Disease

为了揭示心脏瓣膜病中线粒体凋亡调控因子与microRNA的相互作用,本研究检测了心脏瓣膜病患者瓣膜组织病变部位和周围健康组织中的线粒体凋亡调控因子PUMA、Drp1和ARC的表达,并分析了病变部位的ROS水平和SOD活性,同时考察了miR-214和miR-15a对凋亡因子PUMA和ARC的影响。研究显示,PUMA和Drp1在病变组织中表达水平显著升高,而ARC表达显著降低(p<0.05)。病变组织中ROS水平显著升高,而SOD活性显著降低(p<0.05)。瓣膜组织病变部位的miR-214表达水平显著升高,而miR-15a显著降低(p<0.05)。应用miRNA抑制剂下调miR-214和miR-15a后,PUMA和ARC的表达水平被显著上调(p<0.05)。本研究表明在心脏瓣膜病患者中,miR-214和miR-15a分别靶向ARC和PUMA。

To reveal the interaction between mitochondrial apoptosis regulators and microRNAs in valvular heart disease, this study examined the expression of mitochondrial apoptosis regulators PUMA, Drp1 and ARC in valvular lesions and surrounding healthy tissues of patients with valvular heart disease. The ROS levels and SOD activities in the lesions were analyzed. The effects of miR-214 and miR-15a on the apoptotic factors PUMA and ARC were also investigated. Studies have shown that PUMA and Drp1 expression levels are significantly increased in the lesions, while ARC expression is significantly reduced (p<0.05). The level of ROS in the diseased tissue was significantly increased, while the activity of SOD was significantly decreased (p<0.05). The expression level of miR-214 in the valvular lesions was significantly increased, while miR-15a was significantly decreased (p<0.05). After down-regulation of miR-214 and miR-15a by miRNA inhibitors, the expression levels of PUMA and ARC were significantly up-regulated (p<0.05). It was shown that in patients with valvular heart disease, miR- 214 and miR-15a target ARC and PUMA, respectively.