IL-17、IL-6和IL-10因子在急性冠状动脉综合征的作用机制

Action Mechanism of the Factors of IL-17, IL-6 and IL-10 in Acute Coronary Syndrome

为了探讨IL-17、IL-6和IL-10因子在急性冠状动脉综合征的作用机制,本研究采取了78例接受诊断性导管插入术的患者的血样,并将样品分为AMI、UA和SA3组,通过ELISA实验检测3组患者中IL-10、IL-6和IL-17细胞因子的表达,通过流式细胞术检测Th17和Treg细胞的百分比,最后用RT-PCR的方法检测转录因子Foxp3和RORγt的表达。研究结果表明ACS患者IL-10、IL-6和IL-17细胞因子表达异常,且Th17和Treg细胞百分比失衡,转录因子Foxp3和RORγt的mRNA水平也出现异常。ACS患者存在IL-10、IL-6和IL-17细胞因子表达异常,而细胞因子的异常表达是由Th17/Treg细胞失衡引起的,Th17/Treg细胞失衡同时影响多个重要转录因子的表达,因此在ACS发病中具有重要调控作用。

Investigating the functional mechanism of IL-17, IL-6 and IL-10 factors in acute coronary syndrome. We took blood samples from 78 patients undergoing diagnostic catheterization and divided the samples into three groups: AMI, UA, and SA. The blood samples were tested for IL-10, IL-6, and IL-17 by ELISA.The expression of cytokines was detected by flow cytometry. The expression of transcription factors Foxp3 and RORγt was detected by RT-PCR. The results showed that the expression of IL-10, IL-6 and IL-17 cytokines was abnormal in ACS patients, the percentage of Th17 and Treg cells was imbalanced, and the mRNA levels of transcription factors Foxp3 and RORγt were also abnormal. The expression of IL-10, IL-6 and IL-17 cytokines is abnormal in ACS patients, while the abnormal expression of cytokines is caused by the imbalance of Th17/Treg cells. The imbalance of Th17/Treg cell affects the expression of multiple important transcription factors and therefore plays an important regulatory role in the pathogenesis of ACS.