PEG引发紫花苜蓿种子的最优处理方案

Optimum treatment scheme for alfalfa seeds primed with PEG

为了筛选出PEG引发紫花苜蓿(Medicago sativa)种子的最优处理方案,以不同的PEG浓度(5%、10%、15%)、引发时间(12、24、36h)以及引发温度(10、15、20℃)为试验因素,采用正交设计筛选引发紫花苜蓿种子萌发的最优处理方案,并根据生理指标的测定来进一步筛选最优组合。结果表明,正交设计优化PEG引发紫花苜蓿种子处理方案中的最优组合是A2B2C3,即紫花苜蓿种子在温度为20℃的条件下,在浓度为10%的PEG-6000溶液中引发24h后,其发芽速度可显著提高,且种子内部的超氧化物歧化酶、过氧化物酶、过氧化氢酶的活性增强。

In order to determine the best seed priming scheme for alfalfa, the seeds were primed in different concentrations of polyethylene glycol (PEG)(5%, 10%, and 15%) with different durations (12 h, 24 h and 36 h) and temperatures (10, 15, and 20 ℃). The orthogonal design was used to determine the best treatment plan, based on the alfalfa seed germination conditions and the physiological index. The results show that the best treatment scheme was A2B2C3, which represents priming with 10% PEG at 20 ℃ for 24 h. The germination speed of seeds improved significantly, and the activity of superoxide dismutase (SOD), the peroxidase (POD), and catalase (CAT) increased.